Mediator-Microorganism Interaction in Microbial Solar Cell: a Fluo-Electrochemical Insight
Mediator-Microorganism Interaction in Microbial Solar Cell: a Fluo-Electrochemical Insight
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DOI：
10.1021/acs.analchem.9b05808
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发表时间：
2020-06-02
影响因子：
7.4
通讯作者：
Lemaitre, Frederic
中科院分区：
文献类型：
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作者：
Beauzamy, Lena;Delacotte, Jerome;Lemaitre, Frederic
主要依靠光合生物体利用的微生物太阳能电池是用于太阳能发电的光伏发电的有前途的替代品。通过这种方式，我们提出了一种涉及电化学和荧光技术的新方法。耦合设置电脉冲幅度调制（“e-PAM”）能够同时记录光合作用链产生的光电流和荧光信号。该方法通过绿藻莱茵衣藻悬浮液与外源氧化还原介体（2,6-二氯苯醌；DCBQ）的相互作用进行了验证。可以长时间监测光合链事件（PSII 光化学产量、猝灭）和提取的电力之间的平衡。更具体地，DCBQ引起的非光化学猝灭反映了光电流。因此，这种设置有助于实时区分电子捕获和醌引起的一些副作用。因此，它为未来致力于选择实验条件（氧化还原介体、光合生物等）以找到最佳电子提取的分析铺平了道路。
Microbial solar cells that mainly rely on the use of photosynthesic organisms are a promising alternative to photo-voltaics for solar electricity production. In that way, we propose a new approach involving electrochemistry and fluorescence techniques. The coupled setup Electro-Pulse-Amplitude-Modulation ("e-PAM") enables the simultaneous recording of the produced photocurrent and fluorescence signals from the photosynthetic chain. This methodology was validated with a suspension of green alga Chlamydomonas reinhardtii in interaction with an exogenous redox mediator (2,6-dichlorobenzoquinone; DCBQ). The balance between photosynthetic chain events (PSII photochemical yield, quenching) and the extracted electricity can be monitored overtime. More particularly, the nonphotochemical quenching induced by DCBQ mirrors the photocurrent. This setup thus helps to distinguish the electron harvesting from some side effects due to quinones in real time. It therefore paves the way for future analyses devoted to the choice of the experimental conditions (redox mediator, photosynthetic organisms, and so on) to find the best electron extraction.