Ancylostoma ceylanicum, novel etiological agent for traveler's diarrhea-report of four Japanese patients who returned from Southeast Asia and Papua New Guinea.
Ancylostoma ceylanicum, novel etiological agent for traveler's diarrhea-report of four Japanese patients who returned from Southeast Asia and Papua New Guinea.
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DOI：
10.1186/s41182-018-0087-8
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发表时间：
2018
影响因子：
4.5
通讯作者：
Mikasa K
中科院分区：
文献类型：
--
作者：
Yoshikawa M;Ouji Y;Hirai N;Nakamura-Uchiyama F;Yamada M;Arizono N;Akamatsu N;Yoh T;Kaya D;Nakatani T;Kikuchi E;Katanami Y;Satoh K;Maki R;Miyazato Y;Oba Y;Kasahara K;Mikasa K
东南亚地区的国家土壤传播蠕虫（如蛔虫、鞭虫和钩虫）流行率较高[钩虫、美洲钩虫、锡兰钩虫]。最近的分子生物学研究表明，A。ceylanicum是一种人畜共患钩虫，很可能是世界该地区感染人类的第二大流行钩虫种类，而其他人则指出，这种感染不仅对土著人民，而且对来自其他国家的游客都是一种新出现的公共卫生风险。我们最近遇到了四例A。从东南亚和巴布亚新几内亚返回的日本人中的锡兰虫感染。病例1是一名25岁的男性，他在马来西亚的热带雨林中呆了4周，在那里他在第三周出现腹痛和腹泻。双羟萘酸噻嘧啶处理后排出11蠕虫（5条雄性，6条雌性），并鉴定为A。根据形态特征和线粒体细胞色素c氧化酶亚基1（cox 1）基因的DNA序列，病例2为26岁男性，曾在巴布亚新几内亚担任农业海外合作志愿者2年。他当时没有注意到任何症状，尽管在返回后的体检中在粪便样本中检测到鸡蛋。虽然未能成功采集蠕虫，但对卵进行cox 1和核糖体内转录间隔区（ITS）-1和ITS-2基因的DNA分析表明，它们是A.锡兰病例3为一名47岁男性，在老挝人民民主共和国的一个农村住了一个月，从第三周开始出现水样腹泻。通过内窥镜手术和双羟萘酸噻嘧啶处理后，共采集了9蠕虫（3只雄性，6只雌性）。形态学检查和cox 1基因的分子生物学分析表明，它们是A.锡兰病例4为一名27岁男性，参加团体赴印度旅游5天。返回三周后，他出现腹痛和腹泻。在粪便中发现钩虫卵，经培养后发育成幼虫，经鉴定为钩虫。cox 1基因的分子分析。治疗前，在所有病例中均观察到嗜酸性粒细胞增多。A.锡兰虫是东南亚和西太平洋地区旅行者钩虫病的重要病原体。本文的在线版本（10.1186/s41182-018-0087-8）包含补充材料，可供授权用户使用。
Countries in the Southeast Asia region have a high prevalence of soil-transmitted helminth, such as roundworm, whipworm, and hookworms [Ancylostoma duodenale, Necator americanus, Ancylostoma ceylanicum]. Recent molecular-based surveys have revealed that A. ceylanicum, a zoonotic hookworm, is likely the second most prevalent hookworm species infecting humans in that part of the world, while others have noted that this infection is an emerging public health risk not only for indigenous people but also for visitors from other countries. We recently encountered four cases of A. ceylanicum infection in Japanese individuals who returned from Southeast Asia and Papua New Guinea. Case 1 was a 25-year-old male who stayed in a rainforest in Malaysia for 4 weeks, where he developed abdominal pain and diarrhea in the third week. Eleven adult worms (five males, six females) were expelled after treatment with pyrantel pamoate and identified as A. ceylanicum based on morphological characteristics and DNA sequences of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. Case 2 was a 26-year-old male who spent 2 years as an overseas cooperation volunteer for agriculture in Papua New Guinea. He did not note any symptoms at that time, though eggs were detected in feces samples at a medical check-up examination after returning. Although collection of adult worms was unsuccessful, DNA analysis of the eggs for cox1 and the ribosomal internal transcribed spacer (ITS)-1 and ITS-2 genes demonstrated that they were A. ceylanicum. Case 3 was a 47-year-old male who spent 1 month in a rural village in Lao People’s Democratic Republic and began suffering from watery diarrhea from the third week. A total of nine adult worms (three males, six females) were collected by endoscopic procedures and following treatment with pyrantel pamoate. Morphological examination and molecular analyses of the cox1 gene showed that they were A. ceylanicum. Case 4 was a 27-year-old male who participated in group travel to India for 5 days. Three weeks after returning, he developed abdominal pain and diarrhea. Hookworm eggs were found in feces samples and developed into larvae in culture, which were identified as A. ceylanicum based on molecular analysis of the cox1 gene. Eosinophilia was observed in all of the cases prior to treatment. A. ceylanicum should be recognized as an important etiologic pathogen of hookworm diseases in travelers to countries in the Southeast Asia and West Pacific Ocean regions. The online version of this article (10.1186/s41182-018-0087-8) contains supplementary material, which is available to authorized users.