THE ROLE OF ADENYLIC ACID IN THE ACTIVATION OF PHOSPHORYLASE.
THE ROLE OF ADENYLIC ACID IN THE ACTIVATION OF PHOSPHORYLASE.
复制标题
腺苷酸在磷酸化酶激活中的作用。
DOI：
--
复制
发表时间：
1964
影响因子：
11.1
通讯作者：
C. Cori
中科院分区：
文献类型：
--
作者：
E. Helmreich;C. Cori
we are indebted to Dr. H. U. Bergmeyer for a sample of TPN-Na (Lot no. 6313 113) which was stated to be free of 5'-AMP by enzymatic tests. We have also tested this TPN preparation in our assay system, using 0.36 jg charcoal-treated phosphorylase a. With 0.24 umoles TPN per ml, the rate was 0.021 O.D.340 units X min-'. At 5 times this concentration the rate was 0.020 O.D. units X min-'. With 0.24 ,moles TPN, but in the presence of 5 X 10-4 M 5'-AMP, the rate was 0.034. Hence, concentrations of 5'-AMP which could activate phosphorylase a, were not detected in this TPN preparation. Rabbit liver glycogen was purchased from Mann Research Laboratories, Inc. 5'-AMP-8-C14, specific activity 1.5 mc per mmole, was obtained from Tracerlab, Inc. Dowex-1, 5'-AMP, ATP, sodium salts of glucose-i-P and glucose-6-P, Tris-acetate, and 2 times recrystallized egg albumin were purchased from the Sigma Chemical Co. EDTA-Na2 was a product of Geigy Chemical Co., and 2-mercaptoethanol of Eastman Kodak Co. Norit A was from Pfanstiehl Laboratories, Inc. The Norit was washed successively with HCl and then with water until neutral. All other chemicals were ACS grade reagents. Double distilled or deionized H20 was used throughout.
