Differences in bacterial saliva profile between periodontitis patients and a control cohort
Differences in bacterial saliva profile between periodontitis patients and a control cohort
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DOI：
10.1111/jcpe.12190
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发表时间：
2014-02-01
影响因子：
6.7
通讯作者：
Holmstrup, Palle
中科院分区：
文献类型：
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作者：
Belstrom, Daniel;Fiehn, Nils-Erik;Holmstrup, Palle
目的牙周炎是一种多因素疾病，龈下细菌在其发病中起重要作用。本研究的目的是确定牙周炎是否与特征性唾液细菌谱相关。这是通过比较从慢性牙周炎的受试者与唾液从一个控制cohol.Materials和methodsStimulated唾液样本从139慢性牙周炎患者和447个样本从一个控制队列的唾液的细菌配置文件进行了分析，使用人类口腔微生物识别微阵列（HOMIM）。样本中约300种细菌分类群/簇的频率和水平（平均HOMIM值）用作研究参数。使用Mann-Whitney U-检验和Benjamini-Hochberg校正进行多重比较，分析组间分类群/聚类值的差异。主成分分析被用来可视化细菌群落配置文件获得的HOMIM. Results 8个细菌类群，包括假定的牙周病原体微小单胞菌和Filifactoralocis，和4个细菌集群被确定为统计上更频繁，在更高的水平，从牙周炎患者的样本比对照组的样本。这些差异是独立的个人的吸烟status.ConclusionsPeriodontitis是与一个特征性的细菌唾液不同于对照组的配置文件。
AimPeriodontitis is a multifactorial disease in which subgingival bacteria play an important role in the pathogenesis of the disease. The objective of this study was to determine if periodontitis is associated with a characteristic salivary bacterial profile. This was accomplished by comparing the bacterial profile of saliva from subjects with chronic periodontitis with that of saliva from a control cohort.Materials and methodsStimulated saliva samples from 139 chronic periodontitis patients and 447 samples from a control cohort were analysed using the Human Oral Microbe Identification Microarray (HOMIM). Frequency and levels (mean HOMIM-value) of around 300 bacterial taxa/clusters in samples were used as parameters for investigation. Differences at taxon/cluster values between groups were analysed using Mann-Whitney U-test with Benjamini-Hochberg correction for multiple comparisons. Principal component analysis was used to visualize bacterial community profiles obtained by the HOMIM.ResultsEight bacterial taxa, including putative periodontal pathogens as Parvimonas micra and Filifactor alocis, and four bacterial clusters were identified statistically more frequently and at higher levels in samples from periodontitis patients than in samples from the control cohort. These differences were independent of the individuals' smoking status.ConclusionsPeriodontitis is associated with a characteristic bacterial profile of saliva different from that of a control cohort.