Excretable IR-820 for in vivo NIR-II fluorescence cerebrovascular imaging and photothermal therapy of subcutaneous tumor
Excretable IR-820 for in vivo NIR-II fluorescence cerebrovascular imaging and photothermal therapy of subcutaneous tumor
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DOI：
10.7150/thno.31332
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发表时间：
2019-01-01
期刊：
影响因子：
12.4
通讯作者：
Qian, Jun
中科院分区：
文献类型：
--
作者：
Feng, Zhe;Yu, Xiaoming;Qian, Jun
理由：脑血管疾病与恶性肿瘤一起，仍然对人类健康构成巨大威胁。第二近红外（NIR-II，900-1700 nm）荧光生物成像技术以其高空间分辨率和大穿透深度的优势，以及与之相关的基于生物相容性荧光染料的成像引导治疗已成为一种很有前途的治疗诊断方法。建立小鼠颅窗模型、小鼠大脑中动脉阻塞模型和皮下移植膀胱肿瘤模型。采用IR-820辅助近红外荧光显微镜进行近红外荧光脑血管功能成像。结果：IR-820具有相当的近红外荧光强度，在血清中比在水中亮度高。本文基于IR-820辅助近红外荧光显微镜，实现了小鼠脑血管功能的高空间分辨率和大穿透深度的真实的实时和在体成像。结论：IR-820在近红外-Ⅱ光谱区具有较强的荧光强度和良好的光热效应，具有良好的生物相容性和可排泄性，在功能性血管造影和肿瘤治疗诊断方面具有广阔的应用前景。
Rationale: Cerebrovascular diseases, together with malignancies, still pose a huge threat to human health nowadays. With the advantages of its high spatial resolution and large penetration depth, fluorescence bioimaging in the second near-infrared spectral region (NIR-II, 900-1700 nm) and its related imaging-guided therapy based on biocompatible fluorescence dyes have become a promising theranostics method.Methods: The biocompatibility of IR-820 we used in NIR-II fluorescence bioimaging was verified by long-term observation. The model of the mouse with a cranial window, the mouse model of middle cerebral artery occlusion (MCAO) and a subcutaneous xenograft mouse model of bladder tumor were established. NIR-II fluorescence cerebrovascular functional imaging was carried out by IR-820 assisted NIR-II fluorescence microscopy. Bladder tumor was treated by NIR-II fluorescence imaging-guided photothermal therapy.Results: We have found that IR-820 has considerable NIR-II fluorescence intensity, and shows increased brightness in serum than in water. Herein, we achieved real time and in vivo cerebrovascular functional imaging of mice with high spatial resolution and large penetration depth, based on IR-820 assisted NIR-II fluorescence microscopy. In addition, IR-820 was successfully employed for NIR-II fluorescence imaging and photothermal therapy of tumor in vivo, and the subcutaneous tumors were inhibited obviously or eradicated completely.Conclusion: Due to the considerable fluorescence intensity in NIR-II spectral region and the good photothermal effect, biocompatible and excretable IR-820 holds great potentials for functional angiography and cancer theranostics in clinical practice.