Interactions of cellular glycosaminoglycans with plasma fibronectin and collagen.
Interactions of cellular glycosaminoglycans with plasma fibronectin and collagen.
复制标题
细胞糖胺聚糖与血浆纤连蛋白和胶原蛋白的相互作用。
DOI：
10.1016/0304-4165(82)90311-7
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Keller,JM
中科院分区：
文献类型：
--
作者：
Stamatoglou,SC;Keller,JM
通过 Hepes 缓冲盐水（10 mM Hepes 缓冲液中的 150 mM NaCl）中的亲和柱色谱法研究从瑞士小鼠 3T3 和 SV3T3 细胞中分离的代谢放射性标记糖胺聚糖与血浆纤连蛋白和胶原的相互作用。在没有 Ca2+ 和 Mg2+ 的情况下，细胞表面硫酸乙酰肝素糖胺聚糖与血浆纤连蛋白和天然小牛皮肤胶原蛋白结合，在弱酸性 pH 条件下结合达到最大。可以通过提高离子强度来实现洗脱。当暴露于盐浓度梯度时，3T3 和 SV3T3 硫酸乙酰肝素共洗脱。硫酸乙酰肝素蛋白多糖的核心蛋白不与纤连蛋白或胶原蛋白结合。参考标准软骨素 4-硫酸盐和软骨素 6-硫酸盐对硫酸乙酰肝素结合没有影响，而与纤连蛋白或胶原蛋白结合的 90% 硫酸乙酰肝素用肝素洗脱，并用硫酸皮肤素从纤连蛋白中除去 60%。细胞表面硫酸软骨素蛋白多糖仅在酸性 pH 值下与胶原蛋白结合，未观察到与纤连蛋白相互作用。硫酸软骨素蛋白多糖的胰蛋白酶处理减少了与胶原蛋白的结合。细胞表面透明质酸对纤连蛋白或胶原蛋白没有表现出任何亲和力。
The interactions of metabolically radiolabelled glycosaminoglycans, isolated from Swiss mouse 3T3 and SV3T3 cells, with plasma fibronectin and collagen were studied by affinity column chromatography in Hepes-buffered saline (150 mM NaCl in 10 mM Hepes buffer). Cell surface heparan sulfate glycosaminoglycan bound to plasma fibronectin and to native calf skin collagen in the absence of Ca2+and Mg2+with maximal binding at slightly acidic pH. Elution could be effected with elevated ionic strength. 3T3 and SV3T3 heparan sulfates coeluted when exposed to a salt concentration gradient. The core protein of the heparan sulfate proteoglycan did not bind to either fibronectin or collagen. Reference standard chondroitin 4-sulfate and chondroitin 6-sulfate had no effect upon heparan sulfate binding, whereas 90% of the heparan sulfate bound to fibronection or collagen was eluted with heparin and 60% removed from fibronectin with dermatan sulfate. Cell surface chondroitin sulfate proteoglycan bound only to collagen at acidic pH and no interaction was seen with fibronectin. Trypsin treatment of chondroitin sulfate proteoglycan reduced the binding to collagen. Cell surface hyaluronic acid did not display any affinity for either fibronectin or collagen.