Label-free imaging of subcellular features with mid-infrared photothermal microscopy
Label-free imaging of subcellular features with mid-infrared photothermal microscopy
复制标题
使用中红外光热显微镜对亚细胞特征进行无标记成像
DOI：
10.1117/12.2568744
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Samolis, Panagis
中科院分区：
文献类型：
--
作者：
Sander, Michelle Y.;Samolis, Panagis
中红外光热成像是一种结合高灵敏度和增强空间分辨率的新型化学成像方式。在成纤维细胞和组织的亚细胞特征成像和分析有关他们的分子结构，而不需要外源性荧光团在克服中红外激发光束的衍射限制斑点大小的分辨率。通过相敏锁定检测方案，可以检测到热扩散特性的变化，并提供互补的样品表征。
Mid-infrared photothermal imaging is a novel chemical imaging modality that combines high sensitivity with enhanced spatial resolution. Subcellular features in fibroblast cells and tissues are imaged and analyzed with regards to their molecular structures without the need of exogenous fluorophores at a resolution that overcomes the diffraction limited spot size of the mid-infrared excitation beam. With a phase-sensitive lock-in detection scheme, changes in the thermal diffusion properties can be detected and can provide a complementary sample characterization.