Ionic matrix for enhanced MALDI imaging mass spectrometry for identification of phospholipids in mouse liver and cerebellum tissue sections.
Ionic matrix for enhanced MALDI imaging mass spectrometry for identification of phospholipids in mouse liver and cerebellum tissue sections.
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DOI：
10.1021/ac102422b
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发表时间：
2010-10
影响因子：
7.4
通讯作者：
K. Shrivas;T. Hayasaka;Naoko Goto-Inoue;Y. Sugiura;N. Zaima;M. Setou
中科院分区：
文献类型：
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作者：
K. Shrivas;T. Hayasaka;Naoko Goto-Inoue;Y. Sugiura;N. Zaima;M. Setou
离子基质（IM）被认为是多用途的，用于基质辅助激光解吸/电离质谱（MALDI-MS），用于鉴定广泛的生物分子，因为它对各种分析物具有良好的溶解度，与分析物形成均匀的晶体，以及高真空稳定性。利用这些优点，将α-氰基-4-羟基肉桂酸丁胺（CHCAB）和2,5-二羟基苯甲酸丁胺（DHBB）与其他基质的IM性能进行比较，用于标准混合物和小鼠肝组织切片中磷脂的鉴定。结果表明，CHCAB的IM产生较高的信号强度，除了检测肝组织样品表面的磷脂酰胆碱（PC）外，还可以检测到磷脂酰乙醇胺（PE）和磷脂酰丝氨酸（PS）等多种磷脂。CHCAB的IM还用于鉴定存在于小脑不同层中的脂质种类，与DHB基质相比，在小脑不同层中检测到更多的生物分子。此外，该方法的可行性被扩展到分析胰蛋白酶消化细胞色素c，以增加MALDI-MS中的信号强度和肽序列数量。因此，将IM应用于MALDI-MS可以成为高通量、高灵敏度的组织切片生物分子成像的一种有前景的工具。
The ionic matrix (IM) is considered to be versatile for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the identification of a wide range of biomolecules due to its good solubility for a variety of analytes, formation of homogeneous crystals with analytes, and high vacuum stability. When these advantages are exploited, the performance of IM of α-cyano-4-hydroxycinnamic acid butylamine (CHCAB) and 2,5-dihydroxybenzoic acid butylamine (DHBB) was compared with other matrixes for the identification of phospholipids in standard mixtures and mouse liver tissue sections. The results showed that the IM of CHCAB caused higher signal intensity and allowed the detection of a number phospholipids such as phosphatidylethanolamine (PE) and phosphatidylserine (PS) in addition to detection of phosphatidylcholine (PC) on the surface of the liver tissue sample. The IM of CHCAB was also used to identify the species of lipids present in different layers of cerebellum where the greater numbers of biomolecules were detected as compared to DHB matrix. Further, the feasibility of the proposed method was extended for the analysis of tryptic digested cytochrome c for increased signal intensity and number of peptide sequences in MALDI-MS. Thus, the application of IM to MALDI-MS could be a promising tool for imaging biomolecules in tissue sections in high throughput analyses with high sensitivity.