Effects of shoot-applied gibberellin/gibberellin-biosynthesis inhibitors on root growth and expression of gibberellin biosynthesis genes in Arabidopsis thaliana
Effects of shoot-applied gibberellin/gibberellin-biosynthesis inhibitors on root growth and expression of gibberellin biosynthesis genes in Arabidopsis thaliana
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DOI：
10.3117/plantroot.4.4
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发表时间：
2010-01-01
期刊：
影响因子：
0.6
通讯作者：
Satoh, Shinobu
中科院分区：
文献类型：
--
作者：
Bidadi, Haniyeh;Yamaguchi, Shinjiro;Satoh, Shinobu
为了阐明赤霉素（GA）参与拟南芥根的生长调节，研究了地上部施用GA和GA生物合成抑制剂对根的影响。将GA施于拟南芥地上部，对初生根的伸长有一定的促进作用。用GA生物合成抑制剂烯效唑处理芽，也导致初生根伸长的增强，而用烯效唑处理的芽发育迟缓，抽薹延迟。GA3ox和GA20ox的表达分析证实了抑制剂应用于芽后这些基因在根中的上调。结果表明，抑制赤霉素的生物合成可促进根中生物活性赤霉素的产生，并促进根的伸长。
To elucidate the involvement of gibberellin (GA) in the growth regulation of Arabidopsis roots, effects of shoot-applied GA and GA-biosynthesis inhibitors on the root were examined. Applying GA to the shoot of Arabidopsis slightly enhanced the primary root elongation. Treating shoots with uniconazole, a GA biosynthesis inhibitor, also resulted in enhancement of primary root elongation, while shoots treated with uniconazole were stunted and bolting was delayed. Analysis of the expression of GA3ox and GA20ox confirmed the up-regulation of these genes in roots following the inhibitor application to shoots. The results suggest that the inhibition of GA-biosynthesis enhances the production of bioactive GAs in roots and promotes root elongation.