INTERACTION OF KANAMYCIN AND RELATED ANTIBIOTICS WITH LARGE SUBUNIT OF RIBOSOMES AND INHIBITION OF TRANSLOCATION
INTERACTION OF KANAMYCIN AND RELATED ANTIBIOTICS WITH LARGE SUBUNIT OF RIBOSOMES AND INHIBITION OF TRANSLOCATION
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DOI：
10.1016/0006-291x(78)90178-x
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发表时间：
1978-01-01
影响因子：
3.1
通讯作者：
TANAKA, N
中科院分区：
文献类型：
--
作者：
MISUMI, M;NISHIMURA, T;TANAKA, N
采用平衡透析和密孔过滤法研究了[3H]卡那霉素与大肠杆菌核糖体和核糖体亚基的结合。70 S核糖体结合约2个分子，抗生素浓度为10 μ m。浓度越高，浓度越大。每个核糖体亚基都有1个主要结合位点，小核糖体亚基的亲和力大于大核糖体亚基的亲和力。[3H]卡那霉素与核糖体和核糖体亚基的结合可被新霉素或庆大霉素逆转，但不能被链霉素和氯霉素逆转。卡那霉素、新霉素和庆大霉素干扰了[14C]结核菌素o的结合。卡那霉素、新霉素和庆大霉素对N-Ac[乙酰]- ph - trna的易位有明显的抑制作用，而链霉素对N-Ac[乙酰]- ph - trna的易位没有明显的抑制作用。
The binding of [3H]kanamycin to Escherichia coli ribosomes and ribosomal subunits was studied by equilibrium dialysis and Millipore filter methods. The 70 S ribosome bound approximately 2 molecules up to the antibiotic concentration of 10 .mu.M, and more at higher concentrations. Each ribosomal subunit was observed to possess 1 major binding site, and the affinity of the small ribosomal subunit was greater than that of the large subunit. The binding of [3H]kanamycin to ribosomes and ribosomal subunits was reversed by neomycin or gentamicin, but not by streptomycin and chloramphenicol. Kanamycin, neomycin and gentamicin interfered with the binding of [14C] tuberactinomycin O. Translocation of N-Ac[acetyl]-Phe-tRNA was markedly inhibited by kanamycin, neomycin or gentamicin, but not by streptomycin.