Mesenchymal stem cells from human bone marrow and adipose tissue: Isolation, characterization, and differentiation potentialities
Mesenchymal stem cells from human bone marrow and adipose tissue: Isolation, characterization, and differentiation potentialities
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DOI：
10.1007/s10517-005-0430-z
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发表时间：
2005-07-01
影响因子：
0.7
通讯作者：
Buravkova, LB
中科院分区：
文献类型：
--
作者：
Romanov, YA;Darevskava, AN;Buravkova, LB
对培养的人骨髓和脂肪组织（脂肪抽吸物）间充质干细胞进行了比较研究。主要的形态参数，增殖活性，这些细胞的表面和细胞内标志物的表达进行了表征。流式细胞术和组织学染色结果显示，两种细胞均表达CD 105、CD 54、CD 106、HLA-Ⅰ类分子，波形蛋白、ASMA、胶原-1和纤维连接蛋白阳性，而HLA-DR、CD 117和造血细胞标志均不表达。细胞在适当的培养条件下分化为脂肪细胞和成骨细胞。在神经诱导条件下孵育导致出现III型β-微管蛋白（神经元分化标记物）染色阳性的细胞群。
Comparative study of cultured human bone marrow and adipose tissue (lipoaspirate) mesenchymal stem cells was carried out. The main morphological parameters, proliferative activity, expression of surface and intracellular markers of these cells were characterized. Flow cyto-fluorometry and histological staining showed that both cell types exhibited similar expression of CD105, CD54, CD106, HLA-I markers, were positively stained for vimentin, ASMA, collagen-1, and fibronectin, but not HLA-DR, CD 117, and hemopoietic cell markers. The cells underwent differentiation into adipocytes and osteoblasts under appropriate conditions of culturing. Incubation under neuroinductive conditions led to the appearance of a cell population positively stained for type III beta-tubulin (neuronal differentiation marker).