Regulation of tyrosine hydroxylase gene expression in IMR-32 neuroblastoma cells by basic fibroblast growth factor and ciliary neurotrophic factor.
Regulation of tyrosine hydroxylase gene expression in IMR-32 neuroblastoma cells by basic fibroblast growth factor and ciliary neurotrophic factor.
复制标题
碱性成纤维细胞生长因子和睫状神经营养因子对 IMR-32 神经母细胞瘤细胞中酪氨酸羟化酶基因表达的调节。
DOI：
10.1046/j.1471-4159.1995.64062404.x
复制
发表时间：
1995
影响因子：
4.7
通讯作者：
McManaman,JL
中科院分区：
文献类型：
--
作者：
Rabinovsky,ED;Ramchatesingh,J;McManaman,JL
利用IMR-32神经母细胞瘤细胞研究了碱性成纤维细胞生长因子（bFGF）和睫状神经营养因子（CNTF）对酪氨酸羟化酶（TH）基因表达的作用。这些细胞与bFGF治疗3天诱导的免疫反应性TH蛋白和TH mRNA的表达水平可检测。相反，CNTF不影响TH的表达，除非bFGF存在。在饱和量的bFGF的存在下，CNTF增加TH蛋白和TH的mRNA水平，是bFGF处理培养物中发现的两到三倍。CNTF对TH表达的影响随着培养时间的增加而减弱，孵育6天后CNTF不再增强TH水平。CNTF对TH的影响需要bFGF作为辅因子是特定的，因为当CNTF与8-（4-氯苯硫基）-环AMP（另一种刺激该细胞系TH发育的药物）共同给药时，不会影响TH，并且不需要bFGF。CNTF刺激胆碱乙酰转移酶的发育。此外，与bFGF共处理降低了CNTF增强胆碱乙酰转移酶的能力。这些结果表明，bFGF和CNTF可以增强TH的表达，bFGF可以改变CNTF对神经递质表型的影响。
The actions of basic fibroblast growth factor (bFGF) and ciliary neurotrophic factor (CNTF) on tyrosine hydroxylase (TH) gene expression were studied using IMR‐32 neuroblastoma cells. Treatment of these cells with bFGF for 3 days induced the expression of detectable levels of immunoreactive TH protein and TH mRNA. In contrast, CNTF did not affect TH expression unless bFGF was present. In the presence of saturating amounts of bFGF, CNTF increased TH protein and mRNA levels of TH two‐ to threefold over those found in bFGF‐treated cultures. The effects of CNTF on TH expression diminished with increasing culture time, and after 6 days of incubation CNTF no longer enhanced TH levels. The requirement for bFGF as cofactor in the effects of CNTF on TH was specific, as CNTF did not affect TH when it was coadministered with 8‐(4‐chlorophenylthio)‐cyclic AMP, another agent that stimulates TH development in this cell line, and bFGF was not required for CNTF to stimulate the development of choline acetyltransferase. Moreover, cotreatment with bFGF reduced the ability of CNTF to enhance choline acetyltransferase. These results demonstrate that bFGF and CNTF can enhance expression of TH and that bFGF can modify the effects of CNTF on neurotransmitter phenotype.