Macrophage elastase (MMP-12) in expanding murine adipose tissue
Macrophage elastase (MMP-12) in expanding murine adipose tissue
复制标题
DOI：
10.1016/j.bbagen.2012.12.024
复制
发表时间：
2013-04-01
影响因子：
3
通讯作者：
Lijnen, H. R.
中科院分区：
文献类型：
--
作者：
Bauters, D.;Van Hul, M.;Lijnen, H. R.
背景资料：基质金属蛋白酶（MMPs）在脂肪组织的发育中起着重要的作用，但关于单个蛋白酶的作用的信息很少。脂肪组织的扩张与巨噬细胞含量的增加有关。巨噬细胞弹性蛋白酶（Macrophage elastase，MMP-12）在巨噬细胞浸润中具有重要作用，它在脂肪组织中诱导促炎作用。方法：在正常饮食或高脂饮食15周的MMP-12缺陷小鼠和野生型对照小鼠的脂肪组织中研究MMP-12的作用。MMP-12缺乏对总体重或皮下（SC）或性腺（GUN）脂肪组织质量无显著影响。脂肪细胞和血管的大小和密度，在SC和CON脂肪组织的肥胖小鼠也相当的MMP-12缺陷和对照小鼠。在SC和GUN脂肪组织中的巨噬细胞浸润不受MMP-12缺乏的影响，但冠状结构（CLS）的量显着降低。MMP-12缺乏不影响SC或GUN脂肪组织的细胞外基质中的弹性蛋白含量。结论：营养诱导肥胖小鼠的脂肪组织质量和组成不受MMP-12缺乏的明显影响，除了明显较低程度的CLS。MMP-12似乎并不是脂肪组织中巨噬细胞浸润所必需的，但有助于垂死脂肪细胞周围CLS的形成。(C)2013爱思唯尔有限公司版权所有。
Background: Matrix metalloproteinases (MMPs) are known to play a role in adipose tissue development, but little information is available on the role of individual proteinases. Expansion of adipose tissue is associated with an increased macrophage content. Macrophage elastase (MMP-12) has an important role in macrophage infiltration, which induces pro-inflammatory effects in adipose tissue.Methods: The role of MMP-12 was investigated in adipose tissues of MMP-12 deficient and wild-type control mice kept on normal chow or on high fat diet for 15 weeks.Results: MMP-12 deficiency had no significant effect on total body weight or on subcutaneous (SC) or gonadal (GUN) adipose tissue mass. Adipocyte and blood vessel size and density in SC and CON adipose tissues of obese mice were also comparable in MMP-12 deficient and control mice. Macrophage infiltration in SC and GUN adipose tissues was not affected by MMP-12 deficiency, but the amount of crown-like structures (CLS) was significantly lower. MMP-12 deficiency did not affect elastin content in the extracellular matrix of SC or GUN adipose tissue.Conclusions: Adipose tissue mass and composition in mice with nutritionally induced obesity was not markedly affected by MMP-12 deficiency, except for an apparently lower degree of CLS.General Significance: MMP-12 does not seem to be essential for macrophage infiltration in adipose tissue, but contributes to the formation of CLS surrounding moribund adipocytes. (C) 2013 Elsevier B.V. All rights reserved.