The Genetic Basis of Morphological Diversity in Domesticated Goldfish
The Genetic Basis of Morphological Diversity in Domesticated Goldfish
复制标题
DOI：
10.1016/j.cub.2020.04.034
复制
发表时间：
2020-06-22
期刊：
影响因子：
9.2
通讯作者：
Furukawa, Takahisa
中科院分区：
文献类型：
--
作者：
Kon, Tetsuo;Omori, Yoshihiro;Furukawa, Takahisa
虽然驯化金鱼品系表现出高度多样化的形态表型，这些表型的遗传基础是知之甚少。在这里，基于对异源四倍体金鱼基因组中的转座元件的分析，我们发现，它的两个亚基因组已经进化不对称，因为在金鱼和普通鲤鱼的祖先的全基因组重复事件。我们对27个金鱼品系和野生金鱼进行了全基因组测序。我们确定了超过6000万个遗传变异，并建立了主要金鱼品系的群体遗传结构。全基因组关联研究和品系特异性变体分析揭示了与几种金鱼表型相关的遗传位点，包括背鳍缺失、长尾、望远镜眼、白化病和心形尾巴。我们的研究结果表明，在金鱼驯化历史中，不对称进化的亚基因组中的累积突变导致了不同表型的产生。这项研究是了解金鱼品系间表型多样性遗传基础的关键资源。
Although domesticated goldfish strains exhibit highly diversified phenotypes in morphology, the genetic basis underlying these phenotypes is poorly understood. Here, based on analysis of transposable elements in the allotetraploid goldfish genome, we found that its two subgenomes have evolved asymmetrically since a whole-genome duplication event in the ancestor of goldfish and common carp. We conducted whole-genome sequencing of 27 domesticated goldfish strains and wild goldfish. We identified more than 60 million genetic variations and established a population genetic structure of major goldfish strains. Genome-wide association studies and analysis of strain-specific variants revealed genetic loci associated with several goldfish phenotypes, including dorsal fin loss, long-tail, telescope-eye, albinism, and heart-shaped tail. Our results suggest that accumulated mutations in the asymmetrically evolved subgenomes led to generation of diverse phenotypes in the goldfish domestication history. This study is a key resource for understanding the genetic basis of phenotypic diversity among goldfish strains.