COVID-19 and the eye: Systemic and laboratory risk factors for retinopathy and detection of tear film SARS-CoV-2 RNA with a triplex RT-PCR assay.
COVID-19 and the eye: Systemic and laboratory risk factors for retinopathy and detection of tear film SARS-CoV-2 RNA with a triplex RT-PCR assay.
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COVID-19和眼睛：视网膜病变的全身和实验室风险因素以及使用三重RT-PCR测定法检测撕裂膜SARS-COV-2 RNA。
DOI：
10.1371/journal.pone.0277301
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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作者：
评估住院COVID-19住院患者视网膜病变和泪膜SARS-CoV-2 RNA的患病率及其检测的相关风险因素。住院的COVID-19患者接受了散瞳眼科检查和眼底照相。通过三重靶点试验评估结膜拭子的SARS-CoV-2 RT-PCR。我们评估了视网膜病变与临床结果、全身危险因素和实验室数据的关系。中位年龄为59.5岁，29例（48%）为女性。60名患者中有12名（20%）观察到与COVID-19相关的视网膜病变。COVID-19视网膜病变患者的中位年龄为51.5岁，而无视网膜病变患者的中位年龄为62.5岁（p = 0.01）。视网膜病变患者的中位BMI为34.3，无视网膜病变患者为30.9（p = 0.04）。60名患者中有15名（25%）在泪膜中检测到SARS-CoV-2 RNA阳性，与患病和住院时间无关。N2基因特别敏感，19只眼睛中有18只（94.7%）显示N2阳性，包括2例α变体阳性患者（B.1.1.7）。在20%因COVID-19住院的患者中观察到视网膜病变。视网膜病变患者更可能是年轻的，比没有视网膜病变的住院患者有更高的BMI。25%的患者在泪膜中检测到SARS-CoV-2 RNA。肥胖和年龄与视网膜病变的关系需要进一步研究。
To assess hospitalized COVID-19 inpatients for the prevalence of retinopathy and tear film SARS-CoV-2 RNA, and associated risk factors for their detection. Hospitalized COVID-19 patients underwent dilated ophthalmic examination and fundus photography. Conjunctival swabs were assessed for SARS-CoV-2 RT-PCR via a triple target assay. We assessed the relationships of retinopathy with clinical outcomes, systemic risk factors and laboratory data. The median age was 59.5 years and 29 (48%) were female. Retinopathy associated with COVID-19 was observed in 12 of 60 patients (20%). The median age of patients with COVID-19 retinopathy was 51.5 compared to 62.5 years in individuals without retinopathy (p = 0.01). Median BMI was 34.3 in patients with retinopathy versus 30.9 in those without retinopathy (p = 0.04). Fifteen of 60 patients (25%) tested SARS-CoV-2 RNA-positive in their tear film without a relationship with timing of illness and hospitalization. The N2 gene was particularly sensitive with 18 of 19 eyes (94.7%) showing N2-positivity, including 2 patients with alpha variant-positivity (B.1.1.7). Retinopathy was observed in 20% of patients hospitalized for COVID-19. Patients with retinopathy were more likely to be younger and have higher BMI than hospitalized patients without retinopathy. Tear film SARS-CoV-2 RNA was detected in 25% of patients. The relationship of obesity and age with retinopathy requires further investigation.