Attachment of adeno-associated virus type 3H to fibroblast growth factor receptor 1
Attachment of adeno-associated virus type 3H to fibroblast growth factor receptor 1
复制标题
DOI：
10.1007/s00705-005-0650-6
复制
发表时间：
2006-03-01
影响因子：
2.7
通讯作者：
Johnson, FB
中科院分区：
文献类型：
--
作者：
Blackburn, SD;Steadman, RA;Johnson, FB
硫酸乙酰肝素蛋白聚糖被认为是2型腺相关病毒（AAV-2）的主要受体。已报道的共受体包括α V β 5整联蛋白、成纤维细胞生长因子受体1（FGFR-1）和肝细胞生长因子（c-Met）。AAV-3与可能的细胞膜受体的相互作用尚未完全确定。在这项研究中，使用检测与病毒感染的竞争的测定、病毒结合抑制测定和斑点印迹，我们显示AAV-3株H与肝素、硫酸乙酰肝素和FGFR-1的附着。这些发现为AAV-3使用的可能受体阵列提供了新的信息。
Heparan sulfate proteoglycan is thought to act as primary receptor for adeno-associated virus type 2 (AAV-2). Reported coreceptors include alpha V beta 5 integrin, fibroblast growth factor receptor 1 (FGFR-1), and hepatocyte growth factor (c-Met). The interaction of AAV type 3 (AAV-3) with possible cell membrane receptors is incompletely defined. In this study, using assays detecting competition with viral infection, virus binding inhibition assays and dot blotting, we show attachment of AAV-3 strain H to heparin, heparan sulfate, and FGFR-1. These findings provide new information on the possible receptor array used by AAV-3.