Combinational Analyses with Multiple Methods Reveal the Existence of Several Forms of Polysialylated Neural Cell Adhesion Molecule in Mouse Developing Brains
Combinational Analyses with Multiple Methods Reveal the Existence of Several Forms of Polysialylated Neural Cell Adhesion Molecule in Mouse Developing Brains
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DOI：
10.3390/ijms21165892
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发表时间：
2020-08-01
影响因子：
5.6
通讯作者：
Sato, Chihiro
中科院分区：
文献类型：
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作者：
Mori, Airi;Yang, Yi;Sato, Chihiro
聚唾液酸（polySia/PSA）是唾液酸的阴离子聚糖聚合物，其主要修饰哺乳动物脑中的神经细胞粘附分子（NCAM）。多聚Sia-NCAM的多聚Sia的质量和数量在正常的脑发育和功能中受到时空调节，并且据报道它们的损伤与疾病有关，例如精神障碍和癌症。因此，精确了解polySia-NCAM结构的状态将导致疾病的诊断，其合适的评估方法是必要的。在这项研究中，开发这些评估方法，结构的polySia-NCAM从小鼠大脑在六个不同的发展阶段进行了分析，通过几个传统的和新开发的方法。这些实验的综合结果清楚地表明，发育中的大脑中存在不同类型的polySia-NCAMs。此外，组合分析被证明是有用的，精确了解的数量和质量的polySia，这可以提供标准的疾病诊断。
Polysialic acid (polySia/PSA) is an anionic glycan polymer of sialic acid, and it mostly modifies the neural cell adhesion molecule (NCAM) in mammalian brains. Quality and quantity of the polySia of the polySia-NCAM is spatio-temporally regulated in normal brain development and functions, and their impairments are reported to be related to diseases, such as psychiatric disorders and cancers. Therefore, precise understanding of the state of polySia-NCAM structure would lead to the diagnosis of diseases for which their suitable evaluation methods are necessary. In this study, to develop these evaluation methods, structures of polySia-NCAM from mouse brains at six different developmental stages were analyzed by several conventional and newly developed methods. Integrated results of these experiments clearly demonstrated the existence of different types of polySia-NCAMs in developing brains. In addition, combinational analyses were shown to be useful for precise understanding of the quantity and quality of polySia, which can provide criteria for the diagnosis of diseases.