A novel member of the rho family of small GTP-binding proteins is specifically required for cytokinesis.
A novel member of the rho family of small GTP-binding proteins is specifically required for cytokinesis.
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DOI：
10.1083/jcb.133.6.1321
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发表时间：
1996-06
期刊：
影响因子：
--
通讯作者：
De Lozanne A
中科院分区：
文献类型：
--
作者：
Larochelle DA;Vithalani KK;De Lozanne A
rho/rac小gtp结合蛋白家族的几个成员在各种亚细胞过程中调节肌动蛋白细胞骨架的分布。我们在这里描述了一种新的rac蛋白，racE，它是细胞分裂（一个肌动球蛋白介导的过程）所特别需要的。racE基因在Dictyostelium细胞分裂所需基因的分子遗传筛选中被分离出来。racE突变体的表型特征表明，racE对任何其他细胞运动事件（包括吞噬、趋化、封顶或发育）都不是必需的。我们的数据为rho样蛋白的基本需求提供了第一个遗传学证据，特别是在细胞质分裂中，并表明这些蛋白在协调细胞质分裂与细胞周期的有丝分裂事件中的作用。
Several members of the rho/rac family of small GTP-binding proteins are known to regulate the distribution of the actin cytoskeleton in various subcellular processes. We describe here a novel rac protein, racE, which is specifically required for cytokinesis, an actomyosin-mediated process. The racE gene was isolated in a molecular genetic screen devised to isolate genes required for cytokinesis in Dictyostelium. Phenotypic characterization of racE mutants revealed that racE is not essential for any other cell motility event, including phagocytosis, chemotaxis, capping, or development. Our data provide the first genetic evidence for the essential requirement of a rho-like protein, specifically in cytokinesis, and suggest a role for these proteins in coordinating cytokinesis with the mitotic events of the cell cycle.