Benzo[a]pyrene diol epoxide I modification of DNA in human skin xenografts.
Benzo[a]pyrene diol epoxide I modification of DNA in human skin xenografts.
复制标题
人皮肤异种移植物中 DNA 的苯并[a]芘二醇环氧化物 I 修饰。
DOI：
10.1111/1523-1747.ep12475997
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Milo,GE
中科院分区：
文献类型：
--
作者：
Yohn,J;Lehman,TA;Kurian,P;Ribovich,M;Milo,GE
Human skin xenografts were established on the subscapular area of skin of nude (nu/nu NIH-Swiss background) mice. When treated with benzo[a]pyrene diol epoxide I (BPDE I), specific carcinogen-DNA adducts were formed. Separation and identification of these adducts by the32P-postlabeling technique indicated that the 7R- and 7S-BPDE I-dpGp adducts were the major adducts. Xenografts pretreated with either allantoin of anthralin showed an increase in the major 7R- and 7S-BPDE I adducts compared to only BPDE I treatment. Likewise, we observed an increase in the quantity of different minor adducts. The ratios between the minor and major adducts in the pretreated grafts remained consistent with the ratio in the grafts treated with BPDE I only. We conclude that these modulators induce cells in the xenograft to enter S phase of the cell cycle. Moreover, we observed that these compounds altered the quantity of the minor carcinogen-DNA adducts without altering the overall ratios between the major 7R- and 7S-BPDE I-dpGp adducts and the minor carcinogen-DNA adducts.
