MITOSIS IN CULTURES OF NEWT TISSUES .4. THE CELL SURFACE IN LATE ANAPHASE AND THE MOVEMENTS OF RIBONUCLEOPROTEIN
MITOSIS IN CULTURES OF NEWT TISSUES .4. THE CELL SURFACE IN LATE ANAPHASE AND THE MOVEMENTS OF RIBONUCLEOPROTEIN
复制标题
DOI：
10.1016/0014-4827(55)90055-0
复制
发表时间：
1955-01-01
影响因子：
3.7
通讯作者：
BOSS, J
中科院分区：
文献类型：
--
作者：
BOSS, J
蝾螈组织培养物中的成纤维细胞被观察到是活的，并且在固定和用May-Griinwald和Giemsa染色剂或甲基绿色和派洛宁的混合物（pH 4.5）染色后。在后期和末期，细胞表面出现短暂的突起或“起泡”;这些不是组织培养所特有的。这些突起在时间上与细胞核的变化有关，在空间上与染色体有关，这表明它们是对染色体释放的物质作出反应而产生的。成纤维细胞的染色表明，核糖核蛋白可能是负责“起泡”的代理商的可能性。
Fibroblasts in cultures of newt tissues were observed alive and after fixation and staining with either May-Griinwald and Giemsa stains, or a mixture of methyl green and pyronine at pH 4.5. In late anaphase and in telophase transient protuberances or "bubbling" appear at the surface of the cell; these are not peculiar to tissue cultures. The protuberances are related temporally to nuclear changes and spatially to the chromosomes in ways that suggest that they arise in response to an agent released by the chromosomes. The staining of the fibroblasts suggests the possibility that a ribonucleoprotein may be the agent responsible for "bubbling".