Band-like arrangement of taste-like sensory cells at the gastric groove: evidence for paracrine communication
Band-like arrangement of taste-like sensory cells at the gastric groove: evidence for paracrine communication
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DOI：
10.3389/fphys.2013.00058
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发表时间：
2013-01-01
影响因子：
4
通讯作者：
Breer, Heinz
中科院分区：
文献类型：
--
作者：
Eberle, Julia Anna-Maria;Richter, Patric;Breer, Heinz
胃肠道中味觉相关成分的发现使人们对化学感官监测乳糜液成分的机制和生理意义产生了越来越大的兴趣。先前的研究表明，位于“胃沟”的刷状细胞是候选感觉细胞，“胃沟”平行于啮齿动物的“极限脊”，“极限脊”是将眼底和体部分开的结构。一种新的切片技术显示，这些细胞以栅栏状的方式排列，形成一条与整个体上皮边界的带状结构。利用转基因PLCβ2启动子-GFP小鼠和特异性抗体，我们证明了大多数细胞表达味觉“II型”细胞的典型信号蛋白Gustducin、PLCβ2和Trpm5。这些分子特征强烈地表明，细胞可能能够感知摄取的食物的营养或非营养成分。由于没有证据表明刷状细胞是内分泌细胞，人们试图探索这种假定的化学感觉细胞如何将信息传递给“效应”细胞。研究发现，大多数细胞表达神经元型一氧化氮合酶(NOS)，提示与邻近细胞存在旁分泌相互作用。此外，它们还表达胆碱乙酰转移酶(ChAT)和囊泡蛋白SNAP25，这表明胆碱能传递的可能性可能与邻近的肠神经纤维有关。
The discovery of taste-related elements within the gastrointestinal tract has led to a growing interest in the mechanisms and physiological significance of chemosensory monitoring of chymus composition. Previous work suggests that brush cells located in the "gastric groove," which parallels the "limiting ridge," a structure in rodents that divides the fundus from the corpus, are candidate sensory cells. A novel sectioning technique revealed that these cells are arranged in a palisade-like manner forming a band which borders the whole length of the corpus epithelium. Using transgenic PLC beta 2 promoter-GFP mice and specific antibodies, we have demonstrated that most of these cells express gustducin, PLC beta 2, and TRPM5; typical signaling proteins of gustatory sensory "type II" cells. These molecular features strongly suggest that the cells may be capable of sensing nutrient or non-nutrient constituents of the ingested food. Since there is no evidence that brush cells are endocrine cells, attempts were made to explore how such putative chemosensory cells might transmit the information to "effector" cells. It was found that most of the cells express the neuronal nitric oxide synthase (NOS) suggesting some paracrine interaction with adjacent cells. Moreover, they also express choline acetyltransferase (ChAT) as well as the vesicular protein SNAP25, indicating the potential for cholinergic transmission, possibly with subjacent enteric nerve fibers.