Biological functions of glucan-binding protein B of Streptococcus mutans
Biological functions of glucan-binding protein B of Streptococcus mutans
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DOI：
10.1111/j.1399-302x.2007.00351.x
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发表时间：
2007-10-01
影响因子：
--
通讯作者：
Ooshima, T.
中科院分区：
文献类型：
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作者：
Fujita, K.;Matsumoto-Nakano, M.;Ooshima, T.
引言：变形链球菌是人类龋齿的主要致病因子。与S.变形蛋白包括葡糖基转移酶、蛋白抗原C和结合葡聚糖的蛋白。目前已鉴定出至少四种葡聚糖结合蛋白（glucan-binding protein，Gbp）：GbpA、GbpB、GbpC和GbpD。GbpB的生物学功能及其在变形链球菌毒力中的作用尚不清楚。变异体仍有待阐明。利用GbpB缺陷突变株（BD 1），我们在本研究中证明了GbpB在S. mutans.Results:BD 1的生长速率低于其他菌株，同时也显示出对吞噬作用不太敏感，并且形成比亲本菌株MT 8148更长的链。结论：GbpB可能在细胞壁的构建中起重要作用，并参与细胞的分离和维持。
Introduction: Streptococcus mutans has been implicated as a major causative agent of dental caries in humans. Bacterial components associated with the adhesion phase of S. mutans include glucosyltransferases, protein antigen C and proteins that bind glucan. At least four glucan-binding proteins (Gbp) have been identified; GbpA, GbpB, GbpC and GbpD.Methods: In our previous study, the contributions of GbpA and GbpC to the virulence of S. mutans were investigated; however, the biological function of GbpB and its role in the virulence of S. mutans remain to be elucidated. Using a GbpB-deficient mutant strain (BD1), we demonstrated in the present study that GbpB has a role in the biology of S. mutans.Results: The growth rate of BD1 was lower than that of other strains, while it was also shown to be less susceptible to phagocytosis and to form longer chains than the parental strain MT8148. In addition, electron microscope observations of the cell surfaces of BD1 showed that the cell-wall layers were obscure.Conclusion: These results suggest that GbpB may have an important role in cell-wall construction and be involved in cell separation and cell maintenance.