Diffusion Coefficients of Biomolecules Using Long-Lived Spin States
Diffusion Coefficients of Biomolecules Using Long-Lived Spin States
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DOI：
10.1021/ja902030k
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发表时间：
2009-06-10
影响因子：
15
通讯作者：
Bodenhausen, Geoffrey
中科院分区：
文献类型：
--
作者：
Ahuja, Puneet;Sarkar, Riddhiman;Bodenhausen, Geoffrey
我们报告了在蛋白质中首次观察到长寿命态（LLS）的寿命T-LLS超过相应的自旋晶格弛豫时间T-1超过6倍。大生物分子的慢扩散系数特性可以通过LLS方法与商业探针上可用的中等脉冲场梯度（PFGs）相结合来确定，因为自旋记忆的延长减少了PFGs持续时间和/或强度的应变。不需要对生物分子进行同位素标记。
We report the first observation of long-lived states (LLS) having lifetimes T-LLS that exceed the corresponding spin-lattice relaxation times T-1 by more than a factor 6 in a protein. Slow diffusion coefficients characteristic of large biomolecules can be determined by combining LLS methods with moderate pulsed field gradients (PFGs) available on commercial probeheads, as the extension of spin memory reduces the strain on the duration and/or strength of the PFGs. No isotope labeling of the biomolecule is necessary.