Assembly of the Bacterial Ribosome with Circularly Permuted rRNA.
Assembly of the Bacterial Ribosome with Circularly Permuted rRNA.
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用循环排列的 rRNA 组装细菌核糖体。
DOI：
10.1101/2024.04.10.588894
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Williamson,JamesR
中科院分区：
文献类型：
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作者：
Dong,Xiyu;Sheng,Kai;Gebert,LucaFR;Aiyer,Sriram;MacRae,IanJ;Lyumkis,Dmitry;Williamson,JamesR
共转录组装是介导翻译的RNA-蛋白质复合物形成的一个组成特征。对于核糖体合成，先前的研究已经表明，rRNA结构域的严格转录顺序在细菌核糖体生物合成期间可能不是强制性的，因为一系列环状排列的rRNA是可行的。在这项工作中，我们报告的结构洞察到组装的细菌核糖体大亚基（LSU）的基础上cryo-EM密度图的中间体，积累在vitroribosome合成过程中使用一组循环排列（CiPer）的rRNA。所观察到的23个解决核糖体大亚基中间体的合奏揭示了保守的组装路线与合作组装块之间的层次结构。从rRNA的环状排列中可以发现，关键结构rRNA螺旋的形成存在复杂的相互依赖性。虽然结构域合成的顺序不是强制性的，但结构域结合的顺序似乎确实以特定的顺序进行，这可能是由于对有效核糖体合成的强大进化压力。这项工作加强了细菌核糖体大亚基的已知组装层次的鲁棒性，并提供了一个连贯的观点，如何有效地组装CiPer rRNA可以在这种情况下理解。
Co-transcriptional assembly is an integral feature of the formation of RNA–protein complexes that mediate translation. For ribosome synthesis, prior studies have indicated that the strict order of transcription of rRNA domains may not be obligatory during bacterial ribosome biogenesis, since a series of circularly permuted rRNAs are viable. In this work, we report the structural insights into assembly of the bacterial ribosome large subunit (LSU) based on cryo-EM density maps of intermediates that accumulate duringin vitroribosome synthesis using a set of circularly permuted (CiPer) rRNAs. The observed ensemble of 23 resolved ribosome large subunit intermediates reveals conserved assembly routes with an underlying hierarchy among cooperative assembly blocks. There are intricate interdependencies for the formation of key structural rRNA helices revealed from the circular permutation of rRNA. While the order of domain synthesis is not obligatory, the order of domain association does appear to proceed with a particular order, likely due to the strong evolutionary pressure on efficient ribosome synthesis. This work reinforces the robustness of the known assembly hierarchy of the bacterial large ribosomal subunit and offers a coherent view of how efficient assembly of CiPer rRNAs can be understood in that context.