Changes in the nuclear lamina composition during early development of Xenopus laevis
Changes in the nuclear lamina composition during early development of Xenopus laevis
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DOI：
10.1016/0092-8674(85)90073-x
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发表时间：
1985-05
期刊：
影响因子：
64.5
通讯作者：
R. Stick;P. Hausen
中科院分区：
文献类型：
--
作者：
R. Stick;P. Hausen
在非洲爪蟾的早期发展过程中的核纤层的蛋白质组成的变化进行了监测。核纤层蛋白LIII是卵母细胞核中唯一存在的核纤层蛋白，作为核纤层蛋白库，用于形成原核和早期卵裂核。它存在于胚胎中直到尾芽阶段。核纤层蛋白L1和L2，，最初发现于成体细胞核中，在发育的特征时期出现。L，首先出现在中囊胚过渡期（MBT），和L，在原肠胚。胰蛋白酶肽分析显示，在胚胎中发现的所有三种核纤层蛋白形式与成体核纤层蛋白相同。在MBT中观察到的LIII和L1的从头合成不依赖于转录，因此必须是由于母体mRNA的激活。这些结果与早期发育期间发生的其他核变化进行了讨论。
Changes in protein composition of the nuclear lamina were monitored during early development in Xenopus. Lamin LIII, the only lamin present in oocyte nuclei, serves as a lamin pool for the formation of pronuclei and early cleavage nuclei. It is present in embryos up to the tail bud stages. Lamins L, and L,,, the lamins originally found in adult cell nuclei, appear at characteristic times in development. L, first appears at the midblastula transition (MBT), and L,, at the gastrula. Tryptic peptide analysis revealed that all three lamin forms found in the embryo are identical with the adult lamins. De novo synthesis of Llll and L,, observed at MBT, is independent of transcription and must therefore be due to activation of maternal mRNAs. These results are discussed in relation to other nuclear changes occurring during early development.