PROTEOLYTIC-ENZYMES FROM TSETSE FLIES, GLOSSINA-MORSITANS AND GLOSSINA-PALPALIS (DIPTERA, GLOSSINIDAE)
PROTEOLYTIC-ENZYMES FROM TSETSE FLIES, GLOSSINA-MORSITANS AND GLOSSINA-PALPALIS (DIPTERA, GLOSSINIDAE)
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DOI：
10.1016/0020-1790(85)90094-0
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
GOODING, RH
中科院分区：
文献类型：
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作者：
CHEESEMAN, MT;GOODING, RH
来自成年 Glossina morsitans morsitans, G. m. 的蛋白水解酶。中央，G. m。 submorsitans、G. palpalis palpalis 和 G. p. gambiensis 和 G. m. 幼虫使用DEAE-纤维素色谱法和Sephadex凝胶过滤分离morsitans，并通过其水解酪蛋白和/或合成底物的能力来表征。 G. morsitans sensu lato 成虫有七种蛋白水解酶：胰蛋白酶、羧肽酶 A、羧肽酶 B、一种胰凝乳蛋白酶样酶 (PVII)、一种胰蛋白酶样酶 (PVI) 和两种氨肽酶 B、一种胰凝乳蛋白酶样酶 (PVII)、一种胰蛋白酶样酶 (PVI) 和两种氨肽酶（AP1 和 AP2）。在成人 G. p. 中发现了一种额外的胰蛋白酶样酶 PVIII。 palpalis 和 G. p.冈比亚。 G. p. 中未检测到 PVII。须须。来自所有类群成虫的同源酶具有相似的色谱特性和相似的分子量。 G. m 中未检测到 PVI 和 PVII。莫西坦幼虫；然而，幼虫拥有一种独特的胰凝乳蛋白酶样酶，PIX。
Proteolytic enzymes from adult Glossina morsitans morsitans, G. m. centralis, G. m. submorsitans, G. palpalis palpalis, and G. p. gambiensis and larvae of G. m. morsitans were separated using DEAE-cellulose chromatography and Sephadex gel-filtration and characterized by their ability to hydrolyse casein and/or synthetic substrates. Adults of G. morsitans sensu lato have seven proteolytic enzymes: trypsin, carboxypeptidase A, carboxypeptidase B, a chymotrypsin-like enzyme (PVII), a trypsin-like enzyme (PVI) and two aminopeptidases B, a chymotrypsin-like enzyme (PVII), a trypsin-like enzyme (PVI) and two aminopeptidases (AP1 and AP2). An additional trypsin-like enzyme, PVIII, was found in adult G. p. palpalis and G. p. gambiensis. PVII was not detected in G. p. palpalis. Homologous enzymes from adults of all taxa have similar chromatographic properties and similar molecular weights. Neither PVI nor PVII were detected in G. m. morsitans larvae; however larvae possess a unique chymotrypsin-like enzyme, PIX.