Abscisic acid-dependent histone demethylation during postgermination growth arrest in Arabidopsis.
Abscisic acid-dependent histone demethylation during postgermination growth arrest in Arabidopsis.
复制标题
DOI：
10.1111/pce.13547
复制
发表时间：
2019-07
期刊：
影响因子：
--
通讯作者：
Jinfeng Wu;Y. Ichihashi;Takamasa Suzuki;Arisa Shibata;K. Shirasu;Nobutoshi Yamaguchi;Toshiro Ito
中科院分区：
文献类型：
--
作者：
Jinfeng Wu;Y. Ichihashi;Takamasa Suzuki;Arisa Shibata;K. Shirasu;Nobutoshi Yamaguchi;Toshiro Ito
萌发后，幼苗在不利的条件下生长受阻，这是使植物能够在恶劣环境中生存的关键适应。植物激素脱落酸(ABA)在这种停滞中起着关键作用。为了阻止生长，依赖于ABA的转录因子以一种灵活和可逆的方式改变基因表达模式。虽然基因表达的调控在生长停滞中起着重要作用，但对ABA反应的表观遗传学机制还不完全清楚。在这里，我们展示了组蛋白去甲基酶JUMONJI-C结构域蛋白30(JMJ30)和JMJ32控制ABA介导的拟南芥生长停滞。在萌发后阶段(萌发后2-3天)，依赖于ABA的转录因子ABA不敏感3(ABI3)激活JMJ30的表达以响应ABA。然后，JMJ30从SNF1相关蛋白激酶2.8(SnRK2.8)启动子中移除抑制组蛋白标记H3赖氨酸27三甲基化(H3K27me3)，从而激活SnRK2.8的表达。SnRK2.8编码一种激活ABI3的激酶，负责JMJ30和JMJ32介导的生长停滞。涉及ABI3转录因子、JMJ组蛋白去甲基酶和SnRK2.8激酶的前馈环在萌发后阶段微调依赖于ABA的生长停滞。我们的发现强调了组蛋白去甲基酶在调节植物对环境的适应中的重要性。
After germination, seedlings undergo growth arrest in response to unfavourable conditions, a critical adaptation enabling plants to survive harsh environments. The plant hormone abscisic acid (ABA) plays a key role in this arrest. To arrest growth, ABA-dependent transcription factors change gene expression patterns in a flexible and reversible manner. Although the control of gene expression has important roles in growth arrest, the epigenetic mechanisms in the response to ABA are not fully understood. Here, we show that the histone demethylases JUMONJI-C domain-containing protein 30 (JMJ30) and JMJ32 control ABA-mediated growth arrest in Arabidopsis thaliana. During the postgermination stage (2-3 days after germination), the ABA-dependent transcription factor ABA-insensitive3 (ABI3) activates the expression of JMJ30 in response to ABA. JMJ30 then removes a repressive histone mark, H3 lysine 27 trimethylation (H3K27me3), from the SNF1-related protein kinase 2.8 (SnRK2.8) promoter, and hence activates SnRK2.8 expression. SnRK2.8 encodes a kinase that activates ABI3 and is responsible for JMJ30- and JMJ32-mediated growth arrest. A feed-forward loop involving the ABI3 transcription factor, JMJ histone demethylases, and the SnRK2.8 kinase fine-tunes ABA-dependent growth arrest in the postgermination phase. Our findings highlight the importance of the histone demethylases in mediating adaptation of plants to the environment.