DIFFERENTIAL ENERGY-REQUIREMENTS FOR PLATELET RESPONSES - A SIMULTANEOUS STUDY OF AGGREGATION, 3 SECRETORY PROCESSES, ARACHIDONATE LIBERATION, PHOSPHATIDYLINOSITOL BREAKDOWN AND PHOSPHATIDATE PRODUCTION
DIFFERENTIAL ENERGY-REQUIREMENTS FOR PLATELET RESPONSES - A SIMULTANEOUS STUDY OF AGGREGATION, 3 SECRETORY PROCESSES, ARACHIDONATE LIBERATION, PHOSPHATIDYLINOSITOL BREAKDOWN AND PHOSPHATIDATE PRODUCTION
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DOI：
10.1042/bj2080009
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发表时间：
1982-01-01
影响因子：
4.1
通讯作者：
DANGELMAIER, CA
中科院分区：
文献类型：
--
作者：
HOLMSEN, H;KAPLAN, KL;DANGELMAIER, CA
不同的能量需求适用于某些血小板反应;这些差异可能是由于方法不当和血小板制备的差异。本文报道了降低ATP利用率对7种血小板反应性的影响。用5-羟基[3 H]色胺、[3 H]-或[14 C]腺嘌呤、[32 P]Pi或[3 H]花生四烯酸盐预标记的细胞与抗霉素A和2-脱氧-D-葡萄糖一起孵育。在孵育期间的不同时间测定凝血酶和胶原蛋白（仅分泌）诱导的血小板反应、代谢ATP水平和腺苷酸能荷（AEC）。血小板聚集在5-15分钟的滞后后被迅速抑制，并且在AEC = 0.55-0.60时具有50%的抑制。从致密颗粒分泌5-羟基[14 C]色胺和ATP + ADP，以及分泌纤维蛋白原和β-来自α-血小板球蛋白在AEC = 0.65-0.70时，颗粒被平行抑制，没有滞后，抑制率为50%。抑制血小板因子4从α-颗粒剂的50%抑制率AEC = 0.70-0.80。[3 H]花生四烯酸的分解和酸性水解酶的分泌被平行抑制，并且在AEC = 0.80-0.87时，在孵育开始时存在抑制，抑制率为50%。这些响应显然具有不同的能量需求，其以聚集<致密颗粒，α-α的顺序增加。颗粒分泌<酸性水解酶分泌、磷脂酰肌醇分解、磷脂酸形成和花生四烯酸释放。代谢抑制剂对磷脂酰肌醇分解的强力抑制表明，需要能量的步骤参与了磷脂酶C的激活。
Different energy requirements are indicated for some platelet responses; these differences could be due to inadequate methodology and differences in platelet preparation. The efect of decreasing ATP availability on 7 platelet responses measured in gel-filtered human platelets are described. The cells, prelabeled with 5-hydroxy[3H]tryptamine, [3H]- or [14C]adenine, [32P]Pi or [3H]arachidonate, were incubated with antimycin A and 2-deoxy-D-glucose. Platelet responses induced by thrombin and collagen (secretion only), level of metabolic ATP and the adenylate energy charge (AEC) were determined at various times during incubation. Platelet aggregation was rapidly inhibited after a lag of 5-15 min and with 50% inhibition at AEC = 0.55-0.60. Secretion of 5-hydroxy[14C]tryptamine and ATP + ADP from dense granules and of fibrinogen and .beta.-thromboglobulin from .alpha.-granules were inhibited in parallel, without a lag and with 50% inhibition at AEC = 0.65-0.70. The inhibition of secretion of platelet factor 4 from the .alpha.-granules followed another pattern with 50% inhibition AEC = 0.70-0.80. Breakdown of [3H]arachidonate and secretion of acid hydrolases were inhibited in parallel, and inhibition was present at the start of incubation with 50% inhibition at AEC = 0.80-0.87. The responses apparently have different energy requirements, which increase in the order aggregation < dense granule, .alpha.-granule secretion < acid hydrolase secretion, phosphatidylinositol breakdown, phosphatidate formation and arachidonate liberation. The powerful inhibition of phosphatidylinositol breakdown by metabolic inhibitors suggests that energy-requiring steps are involved in the activation of phospholipase C.