Direct Electron Transfer-type Bioelectrocatalysis of Peroxidase at Mesoporous Carbon Electrodes and Its Application for Glucose Determination Based on Bienzyme System
Direct Electron Transfer-type Bioelectrocatalysis of Peroxidase at Mesoporous Carbon Electrodes and Its Application for Glucose Determination Based on Bienzyme System
复制标题
介孔碳电极直接电子转移型过氧化物酶生物电催化及其在双酶体系葡萄糖测定中的应用
DOI：
10.2116/analsci.33.839
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发表时间：
2017
影响因子：
1.6
通讯作者：
K. Kano
中科院分区：
文献类型：
--
作者：
H. Xia;Y. Kitazumi;O. Shirai;K. Kano
首次在0.7V下在POD/碳纳米管混合物修饰电极上检测到辣根过氧化物酶（POD）化合物I的非催化直接电子转移（DET）信号。以Ketjen Black（KB）修饰电极为载体，用戊二醛固定POD，对H_2O_2进行电催化还原，获得了良好的DET型生物电催化性能，起始电位为0.65 V（vs. Ag | AgCl | 饱和KCl）而没有任何电极表面改性。将POD和葡萄糖氧化酶（GOD）共固定在KB修饰电极上，构建了一个双酶电极，将GOD的氧化酶反应与POD对H2 O2的DET型生物电催化还原反应耦合起来。葡萄糖的安培检测具有高灵敏度（0.33 ± 0.01 μA cm^−2 μM^−1）和低检测限（S/N = 3时为2 μM）。
Non-catalytic direct electron transfer (DET) signal of Compound I of horseradish peroxidase (POD) was first detected at 0.7 V on POD/carbon nanotube mixture-modified electrodes. Excellent performance of DET-type bioelectrocatalysis was achieved with POD immobilized with glutaraldehyde on Ketjen Black (KB)-modified electrodes for H_2O_2 reduction with an onset potential of 0.65 V ( vs. Ag | AgCl | sat. KCl) without any electrode surface modification. The POD-immobilized KB electrode was found to be suitable for detecting H_2O_2 with a low detection limit (0.1 μM at S/N = 3) at –0.1 V. By co-immobilizing glucose oxidase (GOD) and POD on the KB-modified electrode, a bienzyme electrode was constructed to couple the oxidase reaction of GOD with the DET-type bioelectrocatalytic reduction of H_2O_2 by POD. The amperometric detection of glucose was performed with a high sensitivity (0.33 ± 0.01 μA cm^−2 μM^−1) and a low detection limit (2 μM at S/N = 3).