Nuclear magnetic resonance microscopy of single neurons under hypotonic perturbation
Nuclear magnetic resonance microscopy of single neurons under hypotonic perturbation
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DOI：
10.1152/ajpcell.1996.271.6.c1895
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发表时间：
1996-12-01
影响因子：
5.5
通讯作者：
Blackband, SJ
中科院分区：
文献类型：
--
作者：
Hsu, EW;Aiken, NR;Blackband, SJ
使用 NMR 显微镜定量检查经受 20% 低渗扰动的灌注单个神经元中水的核磁共振 (NMR) 特征。细胞质和细胞核的横向弛豫时间 (T2) 分别增加了 24.0 +/- 8.5%（平均 +/- SE，n = 8）和 29.7 +/- 5.3% (n = 6)，而表观扩散系数 (ADC) 无显着变化。这些发现与完美渗透压计的行为以及公认的分子弛豫和扩散模型一致，并对当前细胞水特性的看法产生了重大影响。此外，结果表明，细胞肿胀期间组织细胞内与细胞外体积比的增加是急性脑缺血中ADC减少的主要机制。这些数据是对经历生理扰动的单细胞的细胞质和细胞核中水的核磁共振特征的首次直接定量测量，并且可能会提高核磁共振成像在各种疾病状态下的诊断能力。
Nuclear magnetic resonance (NMR) characteristics of water in perfused single neurons undergoing a 20% hypotonic perturbation were examined quantitatively using NMR microscopy. The transverse relaxation times (T2) in the cytoplasm and nucleus increased by 24.0 +/- 8.5% (average +/- SE, n = 8) and 29.7 +/- 5.3% (n = 6), respectively, whereas the apparent diffusion coefficients (ADC) showed no significant change. These findings are consistent with the behaviors of a perfect osmometer and with accepted molecular relaxation and diffusion models and have significant impacts on current views of properties of cellular water. Furthermore, the results suggest that the increase of tissue intracellular-to-extracellular volume ratio during cell swelling is the predominant mechanism underlying the ADC reduction in acute brain ischemia. These data are the first direct quantitative measurements of the NMR characteristics of water in the cytoplasm and nucleus of single cells undergoing physiological perturbations and may lead to an improved diagnostic capability for NMR imaging in a variety of disease states.