UV-B-responsive microRNAs in Populus tremula
UV-B-responsive microRNAs in Populus tremula
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DOI：
10.1016/j.jplph.2009.06.011
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Tang, Guiliang
中科院分区：
文献类型：
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作者：
Jia, Xiaoyun;Ren, Ligang;Tang, Guiliang
MicroRNAs(MiRNAs)在转录后水平下调基因表达方面发挥重要作用。通过我们的室内miRNA过滤器阵列的表达谱分析，在黑杨试管苗中鉴定出一组24个UV-B胁迫响应miRNA(13个上调，11个下调)。RNA印迹和定量逆转录聚合酶链式反应(qRT-PCR)分别验证了6个UV-B响应的miRNA及其相应的靶基因的表达。这些miRNAs的预测靶基因编码不同的蛋白质，包括转录因子和植物激素信号相关蛋白。对UV-B响应的miRNAs的启动子分析表明，存在许多与光相关的顺式元件。然而，这些顺式元件不一定是紫外线响应miRNAs启动子所特有的，这表明可能有其他机制参与了UV响应miRNAs的调控。最后，建立了一个模型来描述由UV-B响应的miRNAs介导的潜在调控网络。这些结果为理解miRNAs作为植物对UV-B和其他胁迫反应的普遍调节因子提供了新的见解。(C)2009年爱思唯尔股份有限公司。版权所有。
MicroRNAs (miRNAs) play vital roles in down-regulating gene expression at the post-transcriptional level.. A set of 24 UV-B stress-responsive miRNAs (13 up-regulated and 11 down-regulated) was identified in Populus tremula plantlet by expression profiling with our in-house miRNA filter array. Six of the UV-B-responsive miRNA and their corresponding target genes were verified for their expressions by RNA blotting and quantitative reverse transcription PCR (qRT-PCR), respectively. The predicted target genes for these miRNAs encode diverse proteins including transcription factors and phytohormone signal-related proteins. Promoter analysis of the UV-B-responsive miRNAs revealed the presence of many light-relevant cis-elements. However, these cis-elements were not necessarily specific to the promoters of UV-responsive miRNAs, indicating that other machinery may be involved in the regulation of UV-responsive miRNAs. Finally, a model was developed to describe the potential regulatory networks mediated by the UV-B-responsive miRNAs in P tremula. These results provide new insights into the understanding of miRNAs as ubiquitous regulators in plant response to UV-B and other stresses. (C) 2009 Elsevier GmbH. All rights reserved.