The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy.
The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy.
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DOI：
10.1038/s41598-017-00533-w
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发表时间：
2017-04-03
影响因子：
4.6
通讯作者：
Ranson NA
中科院分区：
文献类型：
--
作者：
Hesketh EL;Meshcheriakova Y;Thompson RF;Lomonossoff GP;Ranson NA
豇豆花叶病毒（CPMV）是一种类似小核糖核酸的植物病毒。除了对 CPMV 作为植物病原体的内在兴趣外，CPMV 在纳米技术等生物技术应用中也具有重要意义。在这里，我们报告了含有 RNA-2 的野生型 CPMV 和自然形成的空 CPMV 衣壳的高分辨率冷冻电子显微镜 (cryo-EM) 图。这些结构的分辨率足以可视化大的氨基酸。我们为每个图谱完善了原子模型，并确定了参与基因组衣壳化的必需氨基酸。这项工作加深了我们对小核糖核酸病毒基因组衣壳化的了解，并将有助于进一步开发 CPMV 作为生物技术工具。
Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these structures is sufficient to visualise large amino acids. We have refined an atomic model for each map and identified an essential amino acid involved in genome encapsidation. This work has furthered our knowledge of Picornavirales genome encapsidation and will assist further work in the development of CPMV as a biotechnological tool.