Comparison of morphological and functional restoration between asymmetric bilayer chitosan and bladder acellular matrix graft for bladder augmentation in a rat model
Comparison of morphological and functional restoration between asymmetric bilayer chitosan and bladder acellular matrix graft for bladder augmentation in a rat model
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不对称双层壳聚糖与膀胱脱细胞基质移植物用于大鼠膀胱扩张术的形态和功能恢复比较
DOI：
10.1039/c7ra07601k
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Lu, Mujun
中科院分区：
文献类型：
--
作者：
Xiao, Dongdong;Wang, Qiong;Lu, Mujun
由于目前支架的局限性和临床试验的不利结果，人们迫切需要合适的支架来促进膀胱重建。本研究的目的是评价一种新型的非对称双层壳聚糖支架与传统的膀胱无细胞基质移植物(BAMG)在大鼠膀胱增大模型中的比较。24只8周龄雄性SD大鼠随机分为壳聚糖支架组、BAMG组和膀胱切开组。分别于第21天和第70天取大鼠膀胱进行膀胱造影术和常规组织学检查。免疫荧光光度法、意识膀胱测定术、实时定量聚合酶链式反应和蛋白质印迹分析在70d时用膀胱进行。与BAMG相比，壳聚糖支架由膜状致密层和海绵状多孔层组成，具有良好的机械强度和柔韧性。壳聚糖组在膀胱造影、血管再生、血管数量及功能恢复等方面均优于BAMG组。与BAMG引起的膀胱顺应性降低相反，在70d时，壳聚糖增强的膀胱容量增加了近1.5倍，与膀胱切除术组的顺应性相当。壳聚糖组血管内皮细胞生长因子和血管内皮生长因子2水平升高，与低氧相关的SDF1α/CXCR4通路激活有关。这些结果表明，非对称双层壳聚糖支架是一种很有前途的膀胱重建支架。
Because of the limitations of current scaffolds and unfavorable results of clinical trials, proper scaffolds facilitating bladder reconstruction are highly desirable. The aim of this study was to evaluate a novel asymmetric bilayer chitosan scaffold compared with conventional bladder acellular matrix graft (BAMG) in a rat model of bladder augmentation. Twenty-four 8 week-old male Sprague-Dawley rats were randomly assigned to the chitosan scaffold, BAMG and cystotomy groups. The rats' bladders were sampled for cystography and routine histological examination at 21 and 70 days. Immunofluorescence photometry, conscious cystometry, quantitative real-time polymerase chain reaction, and western blot analyses were performed using bladders at 70 days. Compared with BAMG, the chitosan scaffold consisted of a membrane-like compact layer and a sponge-like porous layer with an excellent combination of mechanical strength and flexibility. The chitosan group showed better performances than the BAMG group in radiographic cystography, smooth muscle regeneration, blood vessel numbers and functional restoration. In contrast to reduced bladder compliance induced by BAMG, bladder augmented by chitosan displayed nearly 1.5-fold increased bladder capacity with comparable compliance to that of the cystotomy group at 70 days. The chitosan group exhibited higher levels of VEGF and VEGFR2, associated with the activation of the hypoxia-related SDF-1α/CXCR4 pathway. These results suggested that the asymmetric bilayer chitosan scaffold is a promising scaffold for bladder reconstruction.