Combining conventional QTL analysis and whole-exome capture-based bulk-segregant analysis provides new genetic insights into tuber sprout elongation and dormancy release in a diploid potato population.
Combining conventional QTL analysis and whole-exome capture-based bulk-segregant analysis provides new genetic insights into tuber sprout elongation and dormancy release in a diploid potato population.
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DOI：
10.1038/s41437-021-00459-0
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发表时间：
2021-09
期刊：
影响因子：
3.8
通讯作者：
Bryan GJ
中科院分区：
文献类型：
--
作者：
Sharma SK;McLean K;Colgan RJ;Rees D;Young S;Sønderkær M;Terry LA;Turnbull C;Taylor MA;Bryan GJ
块茎休眠和发芽是商业上重要的马铃薯性状，因为长期块茎储存是确保全年可用性所必需的。块茎在贮藏过程中过早的休眠解除和发芽生长会导致产品质量的显著劣化。此外，欧洲已撤回主要的化学发芽抑制剂氯苯胺灵，因此需要采取其他办法控制发芽。培育休眠期较长和发芽生长较慢的马铃薯品种是一个理想的目标，尽管这必须受到马铃薯种薯工业的需要的限制，因为马铃薯种薯工业需要打破休眠和发芽活力以快速出苗。我们已经进行了详细的遗传分析块茎芽生长使用二倍体马铃薯人口来自两个高度杂合的父母。评价了采用常规QTL分析与使用新型马铃薯全外显子组捕获（WEC）平台的组合批量分离分析（BSA）相关联的双重方法。在两个连续的生长季节的6个时间点，对块茎在储存中的发芽生长进行评估。遗传分析表明，主QTL的存在下，5条染色体上，其中几个是一致的，在两个生长季节。此外，对显示极端芽生长表型的表型原液进行WEC测序以进行BSA。BSA和WEC相结合的方法证实了QTL的位置，并缩小了相关的基因组区域，同时还确定了新的QTL进行进一步研究。总的来说，我们的研究结果揭示了块茎发芽和发芽生长的非常复杂的遗传结构，这对马铃薯和其他长期储存至关重要的根、鳞茎和块茎作物都有影响。
Tuber dormancy and sprouting are commercially important potato traits as long-term tuber storage is necessary to ensure year-round availability. Premature dormancy release and sprout growth in tubers during storage can result in a significant deterioration in product quality. In addition, the main chemical sprout suppressant chlorpropham has been withdrawn in Europe, necessitating alternative approaches for controlling sprouting. Breeding potato cultivars with longer dormancy and slower sprout growth is a desirable goal, although this must be tempered by the needs of the seed potato industry, where dormancy break and sprout vigour are required for rapid emergence. We have performed a detailed genetic analysis of tuber sprout growth using a diploid potato population derived from two highly heterozygous parents. A dual approach employing conventional QTL analysis allied to a combined bulk-segregant analysis (BSA) using a novel potato whole-exome capture (WEC) platform was evaluated. Tubers were assessed for sprout growth in storage at six time-points over two consecutive growing seasons. Genetic analysis revealed the presence of main QTL on five chromosomes, several of which were consistent across two growing seasons. In addition, phenotypic bulks displaying extreme sprout growth phenotypes were subjected to WEC sequencing for performing BSA. The combined BSA and WEC approach corroborated QTL locations and served to narrow the associated genomic regions, while also identifying new QTL for further investigation. Overall, our findings reveal a very complex genetic architecture for tuber sprouting and sprout growth, which has implications both for potato and other root, bulb and tuber crops where long-term storage is essential.
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影响因子：
5.4
作者：
Hackett, Christine A.;Bradshaw, John E.;Bryan, Glenn J.
通讯作者：
Bryan, Glenn J.
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
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作者：
Kloosterman, Bjorn;Abelenda, Jose A.;Bachem, Christian W. B.
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Bachem, Christian W. B.
使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
30.8
作者：
通讯作者：
--
影响因子：
1.5
作者：
Bisognin, D. A.;Manrique-Carpintero, N. C.;Douches, D. S.
通讯作者：
Douches, D. S.