Optimization of a nanotechnology based antimicrobial platform for food safety applications using Engineered Water Nanostructures (EWNS).
Optimization of a nanotechnology based antimicrobial platform for food safety applications using Engineered Water Nanostructures (EWNS).
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DOI：
10.1038/srep21073
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发表时间：
2016-02-15
影响因子：
4.6
通讯作者：
Demokritou P
中科院分区：
文献类型：
--
作者：
Pyrgiotakis G;Vedantam P;Cirenza C;McDevitt J;Eleftheriadou M;Leonard SS;Demokritou P
最近开发了一种利用工程水纳米结构（EWNS）的无化学物质、基于纳米技术的抗菌平台。EWNS具有高表面电荷，携带活性氧（ROS），可以与一系列微生物（包括食源性病原体）相互作用并使其失活。本研究表明，在合成过程中，它们的性质可以被微调和优化，以进一步提高它们的抗菌潜力。开发了基于实验室的EWNS平台，通过修改合成参数对EWNS性能进行微调。采用最先进的分析方法对EWNS的特性（电荷、大小和ROS含量）进行表征。此外，将有机葡萄番茄表面接种大肠杆菌、肠沙门氏菌、无辜性李斯特菌、副偶发分枝杆菌和酿酒酵母等与食品相关的微生物，评估了它们的微生物失活潜力。本文的研究结果表明，在合成过程中可以对EWNS的性质进行微调，从而使其失活效率提高数倍。更具体地说，表面电荷增加了四倍，ROS含量增加了。微生物去除率与微生物有关，在暴露于40000 #/cm3的EWNS气溶胶剂量45分钟后，其去除率在1.0至3.8 log之间。
A chemical free, nanotechnology-based, antimicrobial platform using Engineered Water Nanostructures (EWNS) was recently developed. EWNS have high surface charge, are loaded with reactive oxygen species (ROS), and can interact-with, and inactivate an array of microorganisms, including foodborne pathogens. Here, it was demonstrated that their properties during synthesis can be fine tuned and optimized to further enhance their antimicrobial potential. A lab based EWNS platform was developed to enable fine-tuning of EWNS properties by modifying synthesis parameters. Characterization of EWNS properties (charge, size and ROS content) was performed using state-of-the art analytical methods. Further their microbial inactivation potential was evaluated with food related microorganisms such as Escherichia coli, Salmonella enterica, Listeria innocua, Mycobacterium parafortuitum, and Saccharomyces cerevisiae inoculated onto the surface of organic grape tomatoes. The results presented here indicate that EWNS properties can be fine-tuned during synthesis resulting in a multifold increase of the inactivation efficacy. More specifically, the surface charge quadrupled and the ROS content increased. Microbial removal rates were microorganism dependent and ranged between 1.0 to 3.8 logs after 45 mins of exposure to an EWNS aerosol dose of 40,000 #/cm3.