SEQUENCE OF THE LONG TERMINAL REPEAT AND ADJACENT SEGMENTS OF THE ENDOGENOUS AVIAN VIRUS ROUS-ASSOCIATED VIRUS-0
SEQUENCE OF THE LONG TERMINAL REPEAT AND ADJACENT SEGMENTS OF THE ENDOGENOUS AVIAN VIRUS ROUS-ASSOCIATED VIRUS-0
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DOI：
10.1128/jvi.43.1.191-200.1982
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发表时间：
1982-01-01
影响因子：
5.4
通讯作者：
HUGHES, SH
中科院分区：
文献类型：
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作者：
HUGHES, SH
禽流感相关病毒O（RAV-0）是一种内源性鸡病毒，接种于易感的家鸡后不会引起疾病。用分子克隆法克隆了一个具有感染性的未整合的环状RAV-0 DNA，并测定了其长末端重复序列（LTR）和相邻片段的序列。发现LTR的序列与复制缺陷型内源性病毒EV-1的序列非常相似。与EV-1 LTR一样，RAV-0 LTR比禽肉瘤病毒-禽白血病病毒组致癌成员的LTR小（278个碱基对，而不是330个碱基对）。存在显著的同源性。最显著的差异是在LTR的U3区域，在该区域存在一系列致癌病毒中存在的小片段，而这些片段在RAV-0中不存在。LTR的U3区的这些差异可以解释RAV-0和禽白血病病毒的致癌潜力的差异。我还比较了邻近RAV-0 LTR的区域与现有的禽肉瘤病毒序列。. apprx的一段。在RAV-0和劳斯肉瘤病毒的布拉格C株中，LTR右侧200个碱基（朝向gag）几乎相同。位于env基因末端和U3之间的RAV-0片段是长度为190个碱基。实质上，这整个区段存在于劳斯肉瘤病毒的施密特-鲁平A株中的env和src之间。在布拉格C中，该片段的大部分也存在于env和src之间;在布拉格C中，在与env相邻的区域中有40个碱基的明显缺失。在Schmidt-Ruppin A中，而不是在布拉格C中，该片段的大约一半也存在于src和LTR之间。这种安排对src的收购机制有一定的影响。编码env的gp 37部分的区域在RAV-0和Rous肉瘤病毒中似乎非常相似。env最末端的差异意味着RAV-0、Schmidt-Ruppin A和布拉格C gp 37的羧基末端显著不同。这些意见的影响被认为是。
Rous-associated virus O(RAV-0), an endogenous chicken virus, does not cause disease when inoculated into susceptible domestic chickens. An infectious unintegrated circular RAV-0 DNA was molecularly cloned, and the sequence of the long terminal repeat (LTR) and adjacent segments was determined. The sequence of the LTR was found to be very similar to that of replication-defective endogenous virus EV-1. Like the EV-1 LTR, the RAV-0 LTR is smaller (278 base pairs instead of 330) than the LTR of the oncogenic members of the avian sarcoma virus-avian leukosis virus group. There is significant homology. The most striking differences are in the U3 region of the LTR, and in this region there are a series of small segments present in the oncogenic viruses which are absent in RAV-0. These differences in the U3 region of the LTR could account for the differences in the oncogenic potential of RAV-0 and the avian leukosis viruses. I also compared the regions adjacent to the RAV-0 LTR with the available avian sarcoma virus sequences. A segment of .apprx. 200 bases to the right of the LTR (toward gag) is almost identical in RAV-0 and the Prague C strain of Rous sarcoma virus. The segment of RAV-0 which lies between the end of the env gene and U3 is .apprx. 190 bases in length. Essentially this entire segment is present between env and src in the Schmidt-Ruppin A strain of Rous sarcoma virus. Most of this segment is also present betweeen env and src in Prague C; in Prague C there is an apparent deletion of 40 bases in the region adjacent to env. In Schmidt-Ruppin A, but not in Prague C, about half of this segment is also present between src and the LTR. This arrangement has implications for the mechanism by which src was acquired. The region which encoded the gp37 portion of env appears to be very similar in RAV-0 and the Rous sarcoma viruses. Differences at the very end of env imply that the carboxy termini of RAV-0, Schmidt-Ruppin A and Prague C gp37 are significantly different. The implications of these observations are considered.