Identification of Pepper mild mottle virus in commercial bell pepper in Florida
Identification of Pepper mild mottle virus in commercial bell pepper in Florida
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DOI：
10.1094/pdis.2001.85.6.679d
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发表时间：
2001-06-01
期刊：
影响因子：
4.5
通讯作者：
Shuler, K. D.
中科院分区：
文献类型：
--
作者：
Adkins, S.;Lamb, E. M.;Shuler, K. D.
自1997年以来，在佛罗里达的辣椒上多次鉴定出辣椒温和斑驳病毒（PMMoV）。初步观察是在观赏辣椒（Capsicum chinense，C. frutescens和C. annuum）。然而，在1999年和2000年的冬季生长季节，PMMoV检测到几个甜椒（C。annuum）栽培品种在南佛罗里达的商业生产领域。在甜椒植株中观察到的症状随植株年龄和品种而变化，包括幼苗发育不良和叶片起皱和黄色斑点。果实变形（光泽和斑驳），大小略有减少。较老的果实表现出棕色条纹，在一些品种中，成熟时的颜色不理想。在商业甜椒领域的发病率从<1%到30%不等。机械接种指示宿主的症状、病毒相关双链RNA的分析和包涵体形态表明存在病毒。PMMoV通过使用ELISA（Agdia，埃尔克哈特，印第安纳州）的血清学测试来特异性鉴定。据我们所知，这是在佛罗里达首次明确鉴定出PMMoV（2），尽管之前在1964年分离出相同或类似的病毒，并报道为烟草花叶病毒的Samsun潜伏株（1）。这种种传病毒在集中商业辣椒生产地区的发生需要仔细监测，以避免未来的重大损失。H. Greenleaf等人，Phytopathology 54：1367，1964. (2)C.更湿了。Plant Dis.68：597，1984.
Pepper mild mottle virus(PMMoV) has been identified on pepper in Florida several times since 1997. Initial observations were on ornamental peppers (Capsicum chinense, C. frutescens, andC. annuum). However, in the winter growing seasons of 1999 and 2000, PMMoV was detected in several bell pepper (C. annuum) cultivars in commercial production fields in south Florida. Symptoms in bell pepper plants were observed to vary with plant age and cultivar, and included stunting of young plants and puckering and yellow mottling of leaves. Fruit was deformed (lumpy and mottled) and slightly reduced in size. Older fruit exhibited brown streaks and, in some cultivars, an undesirable color at maturity. Incidence in commercial bell pepper fields varied from <1 to 30%. Symptoms on mechanically inoculated indicator hosts, analysis of viral-associated double-stranded RNA, and inclusion body morphology suggested the presence of a tobamovirus. PMMoV was specifically identified by serological testing using ELISA (Agdia, Elkhart, IN). To our knowledge, this is the first definitive identification of PMMoV (2) in Florida, although a prior isolation of the same or a similar virus was made in 1964 and reported as the Samsun latent strain ofTobacco mosaic virus(1). The occurrence of this seedborne virus in an area of intensive commercial pepper production requires careful monitoring to avoid future significant losses.References: (1) W. H. Greenleaf et al. Phytopathology 54:1367, 1964. (2) C. Wetter. Plant Dis. 68:597, 1984.