Identification and Application of Neutralizing Epitopes of Human Adenovirus Type 55 Hexon Protein.
Identification and Application of Neutralizing Epitopes of Human Adenovirus Type 55 Hexon Protein.
复制标题
人腺病毒55型六邻体蛋白中和表位的鉴定及应用
DOI：
10.3390/v7102896
复制
发表时间：
2015-10-27
期刊：
影响因子：
--
通讯作者：
Zhou R
中科院分区：
文献类型：
--
作者：
Tian X;Ma Q;Jiang Z;Huang J;Liu Q;Lu X;Luo Q;Zhou R
人类腺病毒55型（HADV55）是一种新鉴定出的重新出现的急性呼吸道疾病（ARD）病原体，提议在HADV11和HADV14菌株之间对己酮基因进行重组。中和表位的鉴定对于针对HADV55感染的监测和疫苗发育很重要。在这项研究中，HADV55己酮蛋白的四种特异性表位肽，A55R1（残基138至152），A55R2（残基179至187），A55R4，A55R4（残基247至259）（残基247至259）和A555R7（残留物429至443），然后通过多个序列模型，并通过多种序列进行了模型，并进行了模型。酶联免疫吸附测定（ELISA）和中和测试（NT）。最后，将A55R2掺入人腺病毒3（HADV3）中，并成功获得了嵌合腺病毒RAD3A55R2。嵌合RAD3A55R2可以诱导针对HADV3和HADV55的中和抗体。这项当前的研究将有助于新型腺病毒疫苗候选和腺病毒结构分析的发展。
Human adenovirus type 55 (HAdV55) is a newly identified re-emergent acute respiratory disease (ARD) pathogen with a proposed recombination of hexon gene between HAdV11 and HAdV14 strains. The identification of the neutralizing epitopes is important for the surveillance and vaccine development against HAdV55 infection. In this study, four type-specific epitope peptides of HAdV55 hexon protein, A55R1 (residues 138 to 152), A55R2 (residues 179 to 187), A55R4 (residues 247 to 259) and A55R7 (residues 429 to 443), were predicted by multiple sequence alignment and homology modeling methods, and then confirmed with synthetic peptides by enzyme-linked immunosorbent assay (ELISA) and neutralization tests (NT). Finally, the A55R2 was incorporated into human adenoviruses 3 (HAdV3) and a chimeric adenovirus rAd3A55R2 was successfully obtained. The chimeric rAd3A55R2 could induce neutralizing antibodies against both HAdV3 and HAdV55. This current study will contribute to the development of novel adenovirus vaccine candidate and adenovirus structural analysis.