Down-regulation of AMP-activated protein kinase by calorie restriction in rat liver
Down-regulation of AMP-activated protein kinase by calorie restriction in rat liver
复制标题
DOI：
10.1016/j.exger.2007.07.003
复制
发表时间：
2007-11-01
影响因子：
3.9
通讯作者：
Shimokawa, Isao
中科院分区：
文献类型：
--
作者：
To, Kazuo;Yamaza, Haruyoshi;Shimokawa, Isao
AMP-activated protein kinase (AMPK) may act as a key enzyme for metabolic adaptation to calorie restriction (CR) or reduced growth hormone (GH)-insulin-like growth factor (IGF)-1 signaling, an experimental intervention for lifespan extension in animals. We investigated the protein levels of AMPK alpha and a downstream enzyme, acetyl-CoA carboxylase (ACC), by immunoblotting of liver and quadriceps femoris muscle (QFM) extracts from 6-month-old wild-type (W) and GH-suppressed transgenic (Tg) Wistar rats fed ad libitum (AL) or 30% CR diets from 6 weeks of age. A modified alternate-day feeding regimen for CR yielded a fed-fasted cycle in CR rats, and therefore the effects of overnight fasting in W-AL rats were also evaluated. CR decreased threonine-172-phosphorylated AMPK alpha (p-AMPK alpha; an activated form) levels in the liver, whereas the CR-fed-fasted cycle or overnight fasting did not significantly affect the p-AMPK alpha level. In the QFM, the p-AMPK alpha level was slightly elevated in the CR-fasted phase, but greatly increased in the AL-fasted phase. Suppression of GH did not affect the p-AMPK alpha level. The phosphorylated-ACC levels did not alter in parallel with the p-AMPK alpha level, particularly in the liver. The present results suggest that CR down-regulates the AMPK activity in the liver on a long-term basis. (C) 2007 Published by Elsevier Inc.
