Expression of a long variant of CRACR2A that belongs to the Rab GTPase protein family in endothelial cells.
Expression of a long variant of CRACR2A that belongs to the Rab GTPase protein family in endothelial cells.
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DOI：
10.1016/j.bbrc.2014.11.095
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发表时间：
2015-01-02
影响因子：
3.1
通讯作者：
Beech, David J.
中科院分区：
文献类型：
--
作者：
Wilson, Lesley A.;McKeown, Lynn;Tumova, Sarka;Li, Jing;Beech, David J.
CRACR 2A的敲低对内皮细胞中的CRAC通道缺乏影响。CRACR 2A的敲低消耗的蛋白质是CRACR 2A质量的两倍。CRACR 2A的长变体CRACR 2A-L存在于内皮细胞中。CRACR 2A-L是一种以前未被识别的含有EF-手的Rab GTdR。CRACR 2A-L在内皮管形成中具有积极作用。CRACR 2A蛋白在T细胞中被描述为钙释放激活的钙（CRAC）通道的含EF手的调节剂。在这里，我们寻求相关的钙离子进入内皮细胞。出乎意料的是，设计用于消耗CRACR 2A的短干扰RNA对内皮细胞中的CRAC通道没有影响，但减少了具有大约两倍于CRACR 2A质量的蛋白质的丰度。参考基因序列数据表明编码CRACR 2A的C-末端Rab GT3延伸的变体转录物的潜力。全长克隆证明了长变体在内皮细胞中的表达。它被命名为CRACR 2A-L。序列分析表明，它是一个以前未被承认的成员的Rab GTdR家族。它对内皮管的形成有积极的贡献。数据表明，内皮细胞含有CRACR 2A的长变体，其是一种含有EF-手的Rab蛋白，对CRAC通道缺乏影响。
Knockdown of CRACR2A lacks effect on CRAC channels in endothelial cells. Knockdown of CRACR2A depletes a protein twice the mass of CRACR2A. A long variant of CRACR2A, CRACR2A-L, occurs in endothelial cells. CRACR2A-L is a previously unrecognised EF-hand-containing Rab GTPase. CRACR2A-L has a positive role in endothelial tube formation. CRACR2A protein is described in T cells as an EF-hand-containing modulator of calcium-release-activated calcium (CRAC) channels. Here we sought relevance to calcium entry of endothelial cells. Unexpectedly, short interfering RNA designed to deplete CRACR2A had no effect on CRAC channels in endothelial cells but reduced the abundance of a protein with about twice the mass of CRACR2A. Reference to gene sequence data indicated the potential for a variant transcript encoding a C-terminal Rab GTPase extension of CRACR2A. Full-length cloning demonstrated expression of the long variant in endothelial cells. It was designated CRACR2A-L. Sequence analysis suggested it to be a previously unrecognised member of the Rab GTPase family. It made a positive contribution to endothelial tube formation. The data suggest that endothelial cells contain a long variant of CRACR2A which is an EF-hand-containing Rab protein that lacks impact on CRAC channels.
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影响因子：
21.3
作者：
Srikanth, Sonal;Jung, Hea-Jin;Kim, Kyun-Do;Souda, Puneet;Whitelegge, Julian;Gwack, Yousang
通讯作者：
Gwack, Yousang
影响因子：
20.1
作者：
Li J;Cubbon RM;Wilson LA;Amer MS;McKeown L;Hou B;Majeed Y;Tumova S;Seymour VA;Taylor H;Stacey M;O'Regan D;Foster R;Porter KE;Kearney MT;Beech DJ
通讯作者：
Beech DJ
DOI：
10.1016/j.bbrc.2007.03.206
发表时间：
2007-06-08
影响因子：
3.1
作者：
Shintani, Mami;Tada, Minoru;Katada, Toshiaki
通讯作者：
Katada, Toshiaki
影响因子：
64.8
作者：
Feske, S;Gwack, Y;Rao, A
通讯作者：
Rao, A
Orai通道孔特性和刺激门控：来自Orai晶体结构的含义。
影响因子：
7.3
作者：
Rothberg BS;Wang Y;Gill DL
通讯作者：
Gill DL