EFFECTS OF PROSTAGLANDINS ON NA-TRANSPORT IN ISOLATED COLLECTING TUBULES
EFFECTS OF PROSTAGLANDINS ON NA-TRANSPORT IN ISOLATED COLLECTING TUBULES
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DOI：
10.1007/bf00584850
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发表时间：
1978-01-01
影响因子：
4.5
通讯作者：
IMAI, M
中科院分区：
文献类型：
--
作者：
IINO, Y;IMAI, M
在离体灌流的兔皮质和髓质集合管中观察了前列腺素（PG）对钠转运的直接作用。将动物用醋酸脱氧皮质酮（DOCA，lmg kg-1天-1，i.m.）实验前3-6天。在皮质集合小管中，PGE 2（1.2 × 10 - 6）在皮质集合小管中，10-7-2.5倍10-5 M）、E1（1.2 × M）、E2（1.5 × M）、E3（1.5 × M）、E4（1.5 × M）、E5（1.5 × M）、E6（1.5 × M）、E7（1.5 × M）、E8（1.5 × M）、E9（1.5 × M）。10-5 M）和F2. (1.2. times. 10-5 M）引起经前庭电位差（PDt）可逆性降低。但PGE 2（1.2x. 10-5 M），也不加入PGA 2（1.2 × 0.5M）。10-5 M）对PDt有影响。用PGE 2（1.2 × 10 - 6）降低Na净吸收。10-5 M）从8.6 . ±. 1.36至1.5 .+-。1.04 peq cm ~（-1）s ~（-1）（P < 0.02）。在未用DOCA预处理的兔中，净钠吸收从2.73 ± 0.001减少到2.73 ± 0.001。0.74至1.02 ±. 0.74 peq cm ~（-1）s ~（-1）（P < 0.01）。在外髓集合小管中，PGE 2（1.2 × 1.5）在细胞膜上表达。10-5 M）也引起PDt的可逆降低。PGE2，F2 α。和E1通过作用于肾小管周膜来抑制集合小管中的Na吸收。
Direct tubular effects of prostaglandins (PG) on Na transport were examined in isolated cortical and medullary collecting tubules of rabbits perfused in vitro. The animals were treated with deoxycorticosterone acetate (DOCA, 1 mg kg-1 day-1, i.m.) for 3-6 days before experiments. In the cortical collecting tubules PGE2 (1.2 .times. 10-7-2.5 .times. 10-5 M), E1 (1.2 .times. 10-5 M) and F2.alpha. (1.2 .times. 10-5 M) added to the bath caused reversible decreases in transtubular potential difference (PDt). But neither PGE2 (1.2 .times. 10-5 M) added to the perfusate nor PGA2 (1.2 .times. 10-5 M) added to the bath had an effect on PDt. The net Na absorption was decreased with PGE2 (1.2 .times. 10-5 M) added to the bath from 8.6 .+-. 1.36 to 1.5 .+-. 1.04 peq cm-1 s-1 (P < 0.02). In rabbits not pretreated with DOCA, the net Na absorption was reduced from 2.73 .+-. 0.74 to 1.02 .+-. 0.74 peq cm-1 s-1 (P < 0.01). In the outer medullary collecting tubules PGE2 (1.2 .times. 10-5 M) added to the bath also caused a reversible decrease in PDt. PGE2, F2.alpha. and E1 inhibit Na absorption in the collecting tubules by acting on the peritubular membrane.