Differential membrane perturbation caused by the cell penetrating peptide Tp10 depending on attached cargo
Differential membrane perturbation caused by the cell penetrating peptide Tp10 depending on attached cargo
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DOI：
10.1016/j.febslet.2007.04.046
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发表时间：
2007-05-29
期刊：
影响因子：
3.5
通讯作者：
Graslund, Astrid
中科院分区：
文献类型：
--
作者：
Barany-Wallje, Elsa;Gaur, Jugnu;Graslund, Astrid
在携带荧光团钙黄蛋白的大单层囊泡中，研究了转运蛋白的一种变体细胞穿透肽Tp10引起的膜渗漏。囊泡由两性离子1-棕榈酰-2-油酰-snglycero-3-phosphocholine组成。当55 kDa链亲和素蛋白附着在Tp10上时，发现膜渗漏显著减少。当附着一个5.4 kDa的肽核酸分子时，膜渗漏与单独Tp10引起的膜渗漏相当。结果表明，直接的膜效应可能导致Tp10单独和较小的复合物的膜易位，而这些效应对较大的货物不起作用。(c) 2007年欧洲生化学会联合会。Elsevier B.V.版权所有。
The membrane leakage caused by the cell penetrating peptide Tp10, a variant of transportan, was studied in large unilamellar vesicles with the entrapped fluorophore calcein. The vesicles were composed of zwitterionic 1-palmitoyl-2-oleoyl-snglycero-3-phosphocholine. A significant decrease in membrane leakage was found when the 55 kDa streptavidin protein was attached to Tp10. When a 5.4 kDa peptide nucleic acid molecule was attached, the membrane leakage was comparable to that caused by Tp10 alone. The results suggest that direct membrane effects may cause membrane translocation of Tp10 alone and of smaller complexes, whereas these effects do not contribute for larger cargoes. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.