Pyrrolnitrin production by an Enterobacter agglomerans strain with a broad spectrum of antagonistic activity towards fungal and bacterial phytopathogens
Pyrrolnitrin production by an Enterobacter agglomerans strain with a broad spectrum of antagonistic activity towards fungal and bacterial phytopathogens
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DOI：
10.1007/s002849900037
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发表时间：
1996-04-01
影响因子：
2.6
通讯作者：
Chet, I
中科院分区：
文献类型：
--
作者：
Chernin, L;Brandis, A;Chet, I
成团肠杆菌的菌株IC 1270先前已被描述为几丁质分解酶复合物的生产者和许多真菌植物病原体的拮抗剂[Chernin等人（1995）Appl. Env. Microbiol. 61：1720-1726]。在这里，我们表明，该菌株还产生一种抗生素，该抗生素通过TLC和HPLC纯化，并通过UV、IR、MS和NMR分析鉴定为吡咯硝基[3-氯-4-（2 '-硝基-3'-氯苯基）吡咯]。纯化的抗生素在体外对许多植物病原细菌和真菌有效。这是第一个证据表明硝吡咯菌素可以由假单胞菌以外的细菌产生，并且一种细菌菌株可以同时产生几丁质分解酶和硝吡咯菌素。几丁质酶和硝吡咯在拮抗作用的组合的可能作用进行了讨论。
Strain IC1270 of Enterobacter agglomerans has been previously described as a producer of a complex of chitinolytic enzymes and as an antagonist of many fungal phytopathogens [Chernin et al. (1995) Appl. Env. Microbiol. 61:1720-1726]. Here we show that this strain also produces an antibiotic that was purified by TLC and HPLC and identified by UV, IR, MS, and NMR analyses as pyrrolnitrin [3-chloro-4-(2'-nitro-3'-chlorophenyl)pyrrole]. The purified antibiotic is efficient against many phytopathogenic bacteria and fungi in vitro. This is the first piece of evidence showing that pyrrolnitrin can be produced by bacteria other than Pseudomonas and that one bacterial strain can simultaneously produce chitinolytic enzymes and pyrrolnitrin. The possible role of a combination of chitinases and pyrrolnitrin in antagonism is discussed.