Preparation and characterization of poly (hydroxy butyrate)/chitosan blend scaffolds for tissue engineering applications.
Preparation and characterization of poly (hydroxy butyrate)/chitosan blend scaffolds for tissue engineering applications.
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DOI：
10.4103/2277-9175.188490
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发表时间：
2016
影响因子：
1
通讯作者：
Sadeghi D
中科院分区：
文献类型：
--
作者：
Karbasi S;Khorasani SN;Ebrahimi S;Khalili S;Fekrat F;Sadeghi D
聚羟基丁酸酯(PHB)是一种生物可降解、生物相容的聚合物，具有良好的力学性能。如果某些性能得到改善，这种聚合物可能会成为一种很有前途的支架材料。本工作制备了新型羟基磷灰石/壳聚糖共混支架作为软骨组织工程的三维基质。将不同质量分数的壳聚糖加入到PHB中，用三氟乙酸溶解。实验设计采用田口统计方法。傅立叶变换红外光谱测试表明，随着壳聚糖含量的增加，共混物中PHB的结晶受到抑制。扫描电子显微镜图像显示，支架表面有一层薄而粗糙的顶层，呈结节状结构，由一层多孔的亚层支撑。支架的体外降解率高于纯PHB支架。含有90%重量的氯化钠和40%的重量的壳聚糖的支架具有最大的降解率。这些结果表明，这些新开发的PHB/壳聚糖共混支架可以作为软骨组织工程的三维基质。
Poly (hydroxy butyrate) (PHB) is a biodegradable and biocompatible polymer with good mechanical properties. This polymer could be a promising material for scaffolds if some features improve. In the present work, new PHB/chitosan blend scaffolds were prepared as a three-dimensional substrate in cartilage tissue engineering. Chitosan in different weight percent was added to PHB and solved in trifluoroacetic acid. Statistical Taguchi method was employed in the design of experiments. The Fourier-transform infrared spectroscopy test revealed that the crystallization of PHB in these blends is suppressed with increasing the amount of chitosan. Scanning electron microscopy images showed a thin and rough top layer with a nodular structure, supported with a porous sub-layer in the surface of the scaffolds. In vitro degradation rate of the scaffolds was higher than pure PHB scaffolds. Maximum degradation rate has been seen for the scaffold with 90% wt. NaCl and 40% wt. chitosan. The obtained results suggest that these newly developed PHB/chitosan blend scaffolds may serve as a three-dimensional substrate in cartilage tissue engineering.