Overexpression of JrAMT2 in walnut (Juglans regia L.) rootstock enhances nitrogen level in grafted wild-type walnut scions
Overexpression of JrAMT2 in walnut (Juglans regia L.) rootstock enhances nitrogen level in grafted wild-type walnut scions
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JrAMT2 在核桃 (Juglans regia L.) 砧木中的过表达可提高嫁接野生型核桃接穗的氮水平
DOI：
10.1016/j.scienta.2021.109928
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发表时间：
2021-04
影响因子：
4.3
通讯作者：
Zhang Qi-xiang
中科院分区：
文献类型：
--
作者：
Liu Hui-jun;Zhang Jia-qi;Hu Heng-kang;Huang You-jun;Xv Chuan-mei;Hu Yuan-yuan;Huang Jian-qin;Leslie Charles A.;Lou He-qiang;Zhang Qi-xiang
氮素是重要的常量营养元素之一，在生理基础上是植物生产力和作物产量的关键限制因子。作为无机氮的主要来源，铵是根系吸收的优先来源，特别是在缺氮植物中，因为其同化需要的能量比硝酸盐少。到目前为止，一些铵转运蛋白（AMT）的基因已被分离和部分特点，从植物以外的核桃。核桃树体大，生长和产量需要充足的氮素。为了降低生产成本，减少环境污染，核桃砧木应提高氮素利用率。本研究从核桃中克隆了一个氨转运蛋白基因，命名为JrAMT2，该基因编码461个氨基酸，分子量为52.39 kDa，在核桃组培苗的各个组织中均有表达，其中在根中表达量最高。过量表达JrAMT2的核桃叶片颜色更深，绿色，氮浓度更高。离体嫁接试验表明，过量表达JrAMT2砧木能显著提高WT接穗的氮素水平。这一结果表明，一个潜在的途径，以开发更好的氮利用砧木核桃和其他树木作物。
Nitrogen is one of the most important essential macronutrients, which acts on a physiological basis as a key limiting factor for plant productivity and crop yield. As the primary source of inorganic nitrogen, ammonium is the preferential source for root uptake, especially in nitrogen-deficient plants, because its assimilation requires less energy than nitrate. To date, several ammonium transporter (AMT) genes have been isolated and partially characterized from plants other than walnut. The growth and yield of walnut trees require abundant nitrogen because of its large size. For the purpose of decreasing production costs and reducing environmental pollution, higher nitrogen utilization of walnut rootstock is desirable. In this study, an ammonium transporter gene, encoding a 52.39 kDa poly-peptide of 461 amino acids, designated JrAMT2, was isolated from walnut.JrAMT2was expressed in all tissues of tissue cultured walnut shoots, with the highest expression in roots. Overexpression ofJrAMT2in walnut shoots resulted in darker green foliage and higher nitrogen concentration. In vitro graft experiments showed overexpressionJrAMT2rootstock can significantly increase the nitrogen level in the WT scion. This result indicates a potential approach to development of better nitrogen utilization rootstocks for walnut and other tree crops.
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影响因子：
--
作者：
F. Mizutani
通讯作者：
F. Mizutani
影响因子：
13.8
作者：
Dongyan Zhao;G. Song
通讯作者：
Dongyan Zhao;G. Song
DOI：
10.17660/actahortic.2014.1058.52
发表时间：
2014-12
期刊：
--
影响因子：
--
作者：
A. Friend;J. Palmer;S. Seymour;R. Diack
通讯作者：
A. Friend;J. Palmer;S. Seymour;R. Diack
DOI：
--
发表时间：
2006
期刊：
Chinese Journal of Spectroscopy Laboratory
影响因子：
--
作者：
Zheng Xian
通讯作者：
Zheng Xian
影响因子：
7.2
作者：
Martinez-Garcia, Pedro J.;Crepeau, Marc W.;Neale, David B.
通讯作者：
Neale, David B.