Muscimol dialysis in the retrotrapezoid nucleus region inhibits breathing in the awake rat.
Muscimol dialysis in the retrotrapezoid nucleus region inhibits breathing in the awake rat.
复制标题
梯形后核区域的蝇蕈醇透析抑制清醒大鼠的呼吸。
DOI：
10.1152/jappl.2000.89.1.153
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Li,A
中科院分区：
文献类型：
--
作者：
Nattie,E;Li,A
在麻醉状态下，斜方后核（RTN）区域的失活显著抑制呼吸和化学感受。在清醒大鼠中，我们透析蝇蕈醇30分钟，可逆地抑制RTN区域的神经元。人工脑脊液透析无效。蝇蕈醇（1或10 mM）在15 min内显著降低潮气量（Vt）（降低16 - 17%）。Vtremained降低50 min或更长时间，90 min后恢复。通气量（Vstece）在15 min内显著降低（降低15 - 20%），然后由于频率增加，在40 min内恢复至基线水平。我们认为，这是对Vt降低的一种代偿性生理反应。耗氧量不变。1 mM组对7%CO2的反应是，绝对V速度和V速度的变化显著降低（降低19 - 22%）。在10 mM组中，对透析的反应包括频率的时间相关性增加和体温的降低，这可能反映了蝇蕈醇的更大传播。在清醒的大鼠中，RTN区域提供了呼吸的一部分紧张性驱动，以及对高碳酸血症的一部分反应。
Under anesthesia, inactivation of the retrotrapezoid nucleus (RTN) region markedly inhibits breathing and chemoreception. In conscious rats, we dialyzed muscimol for 30 min to inhibit neurons of the RTN region reversibly. Dialysis of artificial cerebrospinal fluid had no effect. Muscimol (1 or 10 mM) significantly decreased tidal volume (Vt) (by 16–17%) within 15 min. Vtremained decreased for 50 min or more, with recovery by 90 min. Ventilation (V˙e) decreased significantly (by 15–20%) within 15 min and then returned to baseline within 40 min as a result of an increase in frequency. This, we suggest, is a compensatory physiological response to the reduced Vt. Oxygen consumption was unchanged. In response to 7% CO2in the 1 mM group, absolute V˙eand change in V˙ewere significantly reduced (by 19–22%). In the 10 mM group, the response to dialysis included a time-related increase in frequency and decrease in body temperature, which may reflect greater spread of muscimol. In the awake rat, the RTN region provides a portion of the tonic drive to breathe, as well as a portion of the response to hypercapnia.