Expression of interleukin-8 and monocyte chemotactic protein 1 in women with endometriosis
Expression of interleukin-8 and monocyte chemotactic protein 1 in women with endometriosis
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DOI：
10.1016/j.fertnstert.2007.12.067
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发表时间：
2009-03-01
影响因子：
6.7
通讯作者：
Arici, Aydin
中科院分区：
文献类型：
--
作者：
Ulukus, Murat;Ulukus, E. Cagnur;Arici, Aydin
目的：目的：探讨白细胞介素-8（IL-8）和单核细胞趋化蛋白1（MCP-1）在子宫内膜异位症患者和非子宫内膜异位症患者中的表达和定位。来自子宫内膜异位症女性的异位（n = 24）和同源在位子宫内膜（n = 24）以及来自非子宫内膜异位症女性的子宫内膜（n = 27）用于IL-8和MCP-1的免疫组织化学分析。干预：用抗人IL-8和MCP-1抗体对组织切片进行免疫染色。通过显微镜观察子宫内膜异位症患者在位子宫内膜和异位子宫内膜组织中IL-8和MCP-1在整个月经周期中的存在和定位，并与正常子宫内膜进行比较。s）：在正常子宫内膜中。分泌期样品比增殖期样品表达更高水平的上皮IL-8。增生期和分泌期上皮细胞MCP-1表达相似。子宫内膜异位症患者在位内膜上皮细胞IL-8和MCP-1的表达高于正常对照组。结论：IL-8和MCP-1可能参与了子宫内膜异位症的发病过程。（Fertil Steril（R）2009;91：687-93. (C)2009年，美国生殖医学会（American Society for Reproductive Medicine）
Objective: To investigate the expression and localization of interleukin-8 (IL-8) and monocyte chemotactic protein 1 (MCP-1) in women with and without endometriosis.Design: Comparative immunohistochemical study.Setting: Academic medical center.Patient(s): Ectopic (n = 24) and homologous eutopic endometrium (n = 24) from women with endometriosis and endometrium from women without endometriosis (n = 27) were used for immunohistochemical analysis of IL-8 and MCP-1.Intervention(s): Tissue sections were immunostained with antihuman IL-8 and MCP-1 antibodies.Main Outcome Measure(s): Microscopic evaluation to assess the presence and localization of IL-8 and MCP-1 throughout the menstrual cycle in both eutopic endometrial and endometriotic tissues of women with endometriosis and comparison with normal endometrium.Result(s): In normal endometrium. secretory phase samples expressed higher levels of epithelial IL-8 than in proliferative phase samples. Epithelial MCP-1 expression was similar in both proliferative and secretory phases. Proliferative phase samples showed higher epithelial IL-8 and MCP-1 expressions in eutopic endometrium of women with endometriosis compared with that of normal women. Immunoreactivities of both chemokines were significantly increased in the epithelial cells of ectopic endometrial tissues compared with those of normal endometrium.Conclusion(s): These findings Suggest that IL-8 and MCP-1 may be involved in the pathogenesis of endometriosis. (Fertil Steril (R) 2009;91:687-93. (C) 2009 by American Society for Reproductive Medicine.)