One-pot, two-step transaminase and transketolase synthesis of L-gluco-heptulose from L-arabinose
One-pot, two-step transaminase and transketolase synthesis of L-gluco-heptulose from L-arabinose
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DOI：
10.1016/j.enzmictec.2018.05.006
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发表时间：
2018-09-01
影响因子：
3.4
通讯作者：
Ward, John M.
中科院分区：
文献类型：
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作者：
Bawn, Maria;Subrizi, Fabiana;Ward, John M.
利用生物催化合成源自生物质的高附加值化学构件正在成为未来可持续技术日益重要的应用。这里通过转酮醇酶和转氨酶偶联反应证明了从 L-阿拉伯糖（从甜菜浆中获得的主要单糖）合成更高价值的化学品。源自地热奇球菌和耐辐射奇球菌的耐热转酮醇酶在高温下催化从 L-阿拉伯糖和 β-羟基平水合成 L-葡萄糖-庚酮糖，转化率高。 β-羟基丙酮酸是一种用于转酮酶反应的商业上昂贵的化合物，是由 t-丝氨酸和 α-酮戊二酸通过热稳定性转氨酶（也来自地热奇球菌）原位生成的。对这两个步骤进行了研究，并在一锅系统中实施，以实现 L-葡萄糖庚酮糖的可持续和高效生产。
The use of biocatalysis for the synthesis of high value added chemical building blocks derived from biomass is becoming an increasingly important application for future sustainable technologies. The synthesis of a higher value chemical from L-arabinose, the predominant monosaccharide obtained from sugar beet pulp, is demonstrated here via a transketolase and transaminase coupled reaction. Thermostable transketolases derived from Deinococcus geothermalis and Deinococcus radiodurans catalysed the synthesis of L-gluco-heptulose from L-arabinose and beta-hydroxypynwate at elevated temperatures with high conversions. beta-Hydroxypyruvate, a commercially expensive compound used in the transketolase reaction, was generated in situ from t-serine and alpha-ketoglutaric acid via a thermostable transaminase, also from Deinococcus geothermalis. The two steps were investigated and implemented in a one-pot system for the sustainable and efficient production of L-gluco-heptulose.