Mechanistic Exploration of Visible Light-Activated Carbon/TiO2 Hybrid Dots Damaging Bacterial Cells
Mechanistic Exploration of Visible Light-Activated Carbon/TiO2 Hybrid Dots Damaging Bacterial Cells
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DOI：
10.3390/app12199633
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发表时间：
2022-09
期刊：
影响因子：
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通讯作者：
A. F. Adcock;Weixiong Liang;Peter A. Okonjo;Xiuli Dong;K. Sheriff;Ping Wang;Isaiah S. Ferguson;S. Hwu;Ya-Ping Sun;Liju Yang
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文献类型：
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作者：
A. F. Adcock;Weixiong Liang;Peter A. Okonjo;Xiuli Dong;K. Sheriff;Ping Wang;Isaiah S. Ferguson;S. Hwu;Ya-Ping Sun;Liju Yang
碳/TiO 2混合点（C/TiO 2-Dots）在结构上是通过具有有机部分的纳米级碳域表面附着的TiO 2纳米颗粒（来自市售胶体TiO 2样品的直径约为25 nm），因此相当于各自用许多碳点装饰的单个TiO 2纳米颗粒的混合物。这些暴露于可见光的混合点表现出有效的抗菌性能，类似于在具有相同光活化的纯碳量子点中发现的那些。使用活性氧（ROS）清除剂来“淬灭”抗菌活性的结果也是相似的，这表明了共同的机制起源。在探测抗菌作用的生物学后果的实验中的发现表明，可见光激活的C/TiO 2-Dots对细菌细胞膜造成显着损伤，导致更高的渗透性，与相关的氧化应激导致脂质过氧化，抑制细菌生长。透射电子显微镜（TEM）成像可以更直接地观察到诱导的细菌细胞损伤。进一步发展的混合点平台的各种抗菌应用的机会进行了讨论。
The carbon/TiO2 hybrid dots (C/TiO2-Dots) are structurally TiO2 nanoparticles (in the order of 25 nm in diameter from commercially available colloidal TiO2 samples) surface-attached by nanoscale carbon domains with organic moieties, thus equivalent to hybrids of individual TiO2 nanoparticles each decorated with many carbon dots. These hybrid dots with exposure to visible light exhibit potent antibacterial properties, similar to those found in neat carbon dots with the same light activation. The results from the use of established scavengers for reactive oxygen species (ROS) to “quench” the antibacterial activities, an indication for shared mechanistic origins, are also similar. The findings in experiments on probing biological consequences of the antibacterial action suggest that the visible light-activated C/TiO2-Dots cause significant damage to the bacterial cell membrane, resulting in higher permeability, with the associated oxidative stress leading to lipid peroxidation, inhibiting bacterial growth. The induced bacterial cell damage could be observed more directly in the transmission electron microscopy (TEM) imaging. Opportunities for the further development of the hybrid dots platform for a variety of antibacterial applications are discussed.