Effects of assuming constant optical scattering on measurements of muscle oxygenation by near-infrared spectroscopy during exercise
Effects of assuming constant optical scattering on measurements of muscle oxygenation by near-infrared spectroscopy during exercise
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DOI：
10.1152/japplphysiol.00920.2005
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Barstow, Thomas J.
中科院分区：
文献类型：
--
作者：
Ferreira, Leonardo F.;Hueber, Dennis M.;Barstow, Thomas J.
Ferreira LF，Hueber DM，Barstow TJ.假设恒定光散射对运动过程中近红外光谱法测量肌肉氧合的影响。J Appl Physiol 102：358-367，2007.首次发表于2006年10月5日; doi：10.1152/japplphysio1.00920.2005。本研究的目的是研究假设恒定的约化散射系数（μ '（s））对肌肉氧合反应的影响，以增量运动及其恢复动力学。15名受试者（年龄：24 +/- 5岁）接受了增量自行车运动。频域近红外光谱（NIRS）用于估计脱氧血红蛋白浓度{[deoxy（Hb +/- Mb）]}（其中Mb是肌红蛋白）、氧合血红蛋白浓度{[oxy（Hb+Mb）]}、总Hb浓度（Total[ Hb + Mb]）和组织O-2饱和度（Sti（O2）），结合μ '（s）的连续测量值并假设μ'（s）恒定。当测量mu '（s）时，我们观察到峰值工作率Delta[脱氧（Hb+Mb）]（15.0 +/- 7.8 mu M）、Delta[氧（Hb+Mb）]（-4.8 +/- 5.8 mu M）、Delta总[Hb+Mb]（10.9 +/- 8.4 mu M）和Delta Sti（O2）（-11.8 +/- 4.1%）下NIRS变量的显著变化。假设μ '（s）恒定，峰值工作速率下NIRS变量的变化（P < 0.01 vs.测量mu '（s）），其中Delta[脱氧（Hb+Mb）] = 24.5 +/- 15.6 mu M，Delta[氧（Hb+Mb）] = -9.7 +/- 8.2 mu M，Delta总[Hb+Mb] = 14.8 +/- 8.7 mu M，Δ Sti（O2）= -18.7 +/-8.4%。关于回收动力学，测定μ s和假设恒定μ '（s）之间差异的95%置信区间（CI）较大，表明方法之间的一致性较差。对于描述总体动力学的平均响应时间（MRT），95%置信区间为MRT - [脱氧（Hb+Mb）] = 26.7 s; MRT - [脱氧（Hb+Mb）] = 11.8 s，MRT Delta Sti（O2）= 11.8 s。总之，mu '（s）从轻度运动到峰值运动发生变化。此外，假设一个恒定的μ '（s）导致高估了运动过程中NIRS变量的变化和恢复动力学的扭曲。
Ferreira LF, Hueber DM, Barstow TJ. Effects of assuming constant optical scattering on measurements of muscle oxygenation by near-infrared spectroscopy during exercise. J Appl Physiol 102: 358-367, 2007. First published October 5, 2006; doi: 10.1152/japplphysio1.00920.2005.-The aim of this study was to examine the effects of assuming constant reduced scattering coefficient (mu'(s)) on the muscle oxygenation response to incremental exercise and its recovery kinetics. Fifteen subjects (age: 24 +/- 5 yr) underwent incremental cycling exercise. Frequency domain near-infrared spectroscopy (NIRS) was used to estimate deoxyhemoglobin concentration {[deoxy(Hb +/- Mb)]} (where Mb is myoglobin), oxyhemoglobin concentration {[oxy(Hb+Mb)]}, total Hb concentration (Total[ Hb + Mb]), and tissue O-2 saturation (Sti(O2)), incorporating both continuous measurements of mu'(s) and assuming constant mu'(s) . When measuring mu'(s) , we observed significant changes in NIRS variables at peak work rate Delta[deoxy(Hb+Mb)] (15.0 +/- 7.8 mu M), Delta[oxy(Hb+Mb)] (-4.8 +/- 5.8 mu M), Delta Total[Hb+Mb] (10.9 +/- 8.4 mu M), and Delta Sti(O2) (-11.8 +/- 4.1%). Assuming constant mu'(s) resulted in greater (P < 0.01 vs. measured mu'(s)) changes in the NIRS variables at peak work rate, where Delta[deoxy(Hb+Mb)] = 24.5 +/- 15.6 mu M, Delta[oxy(Hb+Mb)] = -9.7 +/- 8.2 mu M, Delta Total[Hb+Mb] = 14.8 +/- 8.7 mu M, and Delta Sti(O2) = -18.7 +/- 8.4%. Regarding the recovery kinetics, the large 95% confidence intervals (CI) for the difference between those determine measuring mu's and assuming constant mu'(s) suggested poor agreement between methods. For the mean response time (MRT), which describes the overall kinetics, the 95% confidence intervals were MRT - [deoxy(Hb+Mb)] = 26.7 s; MRT - [deoxy(Hb+Mb)] = 11.8 s, and MRT Delta Sti(O2) = 11.8 s. In conclusion, mu'(s) changed from light to peak exercise. Furthermore, assuming a constant mu'(s) led to an overestimation of the changes in NIRS variables during exercise and distortion of the recovery kinetics.